| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38243-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79911 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38029-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98696-50ul | |||
![Western Blot: HNF1 Antibody [NBP1-33596] - Analysis of HNF1a transcriptional activity in hepatocytes following transforming growth factor B (TGFB) treatment. (B) Western blot analysis for HNF1a in protein extracts from hepatocytes in a time-course experiment following TGFB treatment. a-Glyceraldehyde 3-phosphate dehydrogenase was used as loading control. Magnification 20x. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fphar.2019.00942/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HNF1-Antibody-Western-Blot-NBP1-33596-img0023.jpg "<b>Biological Strategies Validation. </b>Western Blot: HNF1 Antibody [NBP1-33596] - Analysis of HNF1a transcriptional activity in hepatocytes following transforming growth factor B (TGFB) treatment. (B) Western blot analysis for HNF1a in protein extracts from hepatocytes in a time-course experiment following TGFB treatment. a-Glyceraldehyde 3-phosphate dehydrogenase was used as loading control. Magnification 20x. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fphar.2019.00942/full) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HNF1 Antibody [NBP1-33596] - TGFB overrides HNF1a constitutive expression. Immunofluorescence analysis of hepatocytes transfected with pLCPX-HNF1aMyc (HNF1) or the empty vector (CTR), treated with TGFB for 24 hr or left untreated (NT). . Cells were stained with anti-E-cadherin (green) or anti-HNF1a antibody (red). Magnification 20x. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fphar.2019.00942/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33596-img0022.jpg)
![Western Blot: HNF1 Antibody [NBP1-33596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HNF1-Antibody-Western-Blot-NBP1-33596-img0018.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP1-33596] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-Paraffin-NBP1-33596-img0003.jpg)
![Western Blot: HNF1 Antibody [NBP1-33596] - Rat tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:1000.](http://images.novusbio.com/fullsize/HNF1-Antibody-Western-Blot-NBP1-33596-img0014.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP1-33596] - detects HNF1 alpha protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.HNF1 alpha stained by HNF1 alpha antibody [N1N3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-Paraffin-NBP1-33596-img0020.jpg)
![Immunoprecipitation: HNF1 Antibody [NBP1-33596] - Immunoprecipitation of HNF1 alpha protein from HepG2 whole cell extracts using 5 ug of HNF1 alpha antibody [N1N3]. Western blot analysis was performed using HNF1 alpha antibody [N1N3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunoprecipitation-NBP1-33596-img0016.jpg)
![Western Blot: HNF1 Antibody [NBP1-33596] - (A) Analysis of CBP/p300 DNA binding activity after TGF beta treatment. qPCR analysis of chromatin immunoprecipitated with anti-CBP/p300 antibody from hepatocytes untreated or treated with TGF beta (for 5 h) was performed. HNF1 alpha consensus regions embedded in the indicated target gene promoters were analyzed. A HNF1 alpha nonbound region of Neurogenin 1 promoter was utilized as negative control. Data are normalized to total chromatin input & background (control immunoprecipitation with IgG) & expressed as % input. Mean ± SEM of qPCR data obtained in triplicate from three independent experiments are reported. (B)In vivo coimmunoprecipitation of HNF1 alpha & CBP/p300 in hepatocytes untreated or treated with TGF beta (for 5 h). Cells were lysed, immunoprecipitated with anti-HNF1 alpha antibody, & then analyzed by Western blotting with the indicated antibodies. As control, the immunoprecipitation with normal goat IgG was performed. TCE, total cell extracts. Densitometric analysis of WB data from three independent experiments is shown. (C) Analysis of HNF1 alpha protein PTMs following TGF beta treatment. Nuclear extracts from control hepatocytes (upper panel) & HNF1 alpha Myc-overexpressing hepatocytes treated for 3 h with TGF beta (lower panel) or left untreated (middle panel). Samples were separated by two-dimensional gel electrophoresis followed by Western blotting with anti-Myc-Tag antibody. HNF1 alpha -specific spots are indicated by the arrow. The appearance of multiple spots in TGF beta -treated hepatocytes can be observed. **p Sample: Paraffin-embedded mouse colon.HNF1 stained by HNF1 antibody [N1N3] (NBP1-33596) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33596_rabbit-polyclonal-hnf1-antibody-91020242021969.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP1-33596] - HNF1 antibody [N1N3] detects HNF1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.HNF1 stained by HNF1 antibody [N1N3] (NBP1-33596) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33596_rabbit-polyclonal-hnf1-antibody-91020242011479.jpg)
![Western Blot: HNF1 Antibody [NBP1-33596] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 antibody [N1N3] (NBP1-33596) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33596_rabbit-polyclonal-hnf1-antibody-910202420122014.jpg)
![Immunocytochemistry/ Immunofluorescence: HNF1 Antibody [NBP1-33596] - HNF1 antibody [N1N3] detects HNF1 protein at nucleus by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HNF1 stained by HNF1 antibody [N1N3] (NBP1-33596) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp1-33596_rabbit-polyclonal-hnf1-antibody-91020242041265.jpg)
![Western Blot: HNF1 Antibody [NBP1-33596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 antibody [N1N3] (NBP1-33596) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33596_rabbit-polyclonal-hnf1-antibody-91020242091424.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: HNF1 Antibody [NBP1-33596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-33596_rabbit-polyclonal-hnf1-antibody-1610202419414518.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-38243] - Staining in human small intestine and lymph node tissues . Corresponding HNF1A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38243-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-38243] - Staining in human small intestine and lymph node tissues . Corresponding HNF1A RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: HNF1 Antibody [NBP2-38243] - Analysis in human cell lines Caco-2 and U2OS using anti-HNF1A antibody. Corresponding HNF1A RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/HNF1-Antibody-Western-Blot-NBP2-38243-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: HNF1 Antibody [NBP2-38243] - Staining of human cell line CACO-2 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38243-img0005.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-38243] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38243-img0008.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-38243] - Staining of human liver shows weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38243-img0009.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-38243] - Staining of human lymph node shows no positivity in lymphoid cells.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38243-img0010.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-38243] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38243-img0011.jpg)
![HNF1 antibody [HL3229] detects HNF1 protein by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HNF1 stained by HNF1 antibody [HL3229] (NBP3-48780) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-48780_rabbit-hnf1-mab-hl3229-immunocytochemistry-immunofluorescence-1411202518393122.jpg "HNF1 antibody [HL3229] detects HNF1 protein by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HNF1 stained by HNF1 antibody [HL3229] (NBP3-48780) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.")
![Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 antibody [HL3229] (NBP3-48780) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48780_rabbit-hnf1-mab-hl3229-western-blot-1411202518313222.jpg)
![<b>Orthogonal Strategies Validation. </b>HNF1 antibody [HL3229] detects HNF1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.HNF1 stained by HNF1 antibody [HL3229] (NBP3-48780) diluted at 1:100.Antigen Retrieval: Tris-EDTA-Citrate acid buffer, pH 7.8, 15 minCorresponding RNA expression data for the same tissues are based on NCBI.](http://images.novusbio.com/fullsize/nbp3-48780_rabbit-hnf1-mab-hl3229-immunohistochemistry-paraffin-1411202518335913.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 antibody [HL3229] (NBP3-48780) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48780_rabbit-hnf1-mab-hl3229-western-blot-141120251836365.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 antibody [HL3229] (NBP3-48780) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48780_rabbit-hnf1-mab-hl3229-western-blot-1411202518345923.jpg)
![HNF1 antibody [HL3229] detects HNF1 protein by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.HNF1 stained by HNF1 antibody [HL3229] (NBP3-48780) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48780_rabbit-hnf1-mab-hl3229-immunohistochemistry-paraffin-141120251838413.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Small Intestine stained with HNF1 Antibody (HNF1A/2087).](http://images.novusbio.com/fullsize/HNF1-Antibody-HNF1A-2087-Immunohistochemistry-Paraffin-NBP2-79777-img0003.jpg "Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Small Intestine stained with HNF1 Antibody (HNF1A/2087).")
![Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Pancreas stained with HNF1 Antibody (HNF1A/2087).](http://images.novusbio.com/fullsize/HNF1-Antibody-HNF1A-2087-Immunohistochemistry-Paraffin-NBP2-79777-img0001.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Pancreas stained with HNF1 Antibody (HNF1A/2087).](http://images.novusbio.com/fullsize/HNF1-Antibody-HNF1A-2087-Immunohistochemistry-Paraffin-NBP2-79777-img0002.jpg)
![Protein Array: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HNF1 Antibody (HNF1A/2087). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/HNF1-Antibody-HNF1A-2087-Protein-Array-NBP2-79777-img0004.jpg)
![Western Blot: HNF1 Antibody (4110) [NBP2-43652] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody (4110) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/HNF1-Antibody-4110-Western-Blot-NBP2-43652-img0003.jpg "Western Blot: HNF1 Antibody (4110) [NBP2-43652] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody (4110) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunohistochemistry-Paraffin: HNF1 Antibody (4110) [NBP2-43652] - Sample: Paraffin-embedded human SW480. HNF1 alpha antibody (4110) diluted at 1:250.](http://images.novusbio.com/fullsize/HNF1-Antibody-4110-Immunohistochemistry-Paraffin-NBP2-43652-img0002.jpg)
![Western Blot: HNF1 Antibody (4110) [NBP2-43652] - Analysis of 30 ug whole cell lysate. A: HepG2 7.5% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/HNF1-Antibody-4110-Western-Blot-NBP2-43652-img0001.jpg)
![Western Blot: HNF1 Antibody [NBP2-32125] - Samples: Whole cell lysate (50 ug) from Hep G2 cells. Antibodies: Affinity purified rabbit anti-HNF1-alpha antibody NBP2-32125 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HNF1-Antibody-Western-Blot-NBP2-32125-img0001.jpg "Western Blot: HNF1 Antibody [NBP2-32125] - Samples: Whole cell lysate (50 ug) from Hep G2 cells. Antibodies: Affinity purified rabbit anti-HNF1-alpha antibody NBP2-32125 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: HNF1 Antibody [NBP2-32125] - Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- HNF1-alpha used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-NBP2-32125-img0003.jpg)
![Immunoprecipitation: HNF1 Antibody [NBP2-32125] - Samples: Whole cell lysate (50 ug) from Hep G2 cells. Antibodies: Affinity purified rabbit anti-HNF1-alpha antibody NBP2-32125 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunoprecipitation-NBP2-32125-img0002.jpg)
![Western Blot: HNF1 Antibody [NBP2-32123] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Hep G2 cells. Antibodies: Affinity purified rabbit anti-HNF1-alpha antibody NBP2-32123 used for IP at 6 ug/mg lysate. HNF1-alpha was also immunoprecipitated by rabbit anti-HNF1-alpha antibodies NBP2-32124, NBP2-32125, NBP2-32126. For blotting immunoprecipitated HNF1-alpha, NBP2-32125 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/HNF1-Antibody-Western-Blot-NBP2-32123-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: HNF1 Antibody [NBP2-32123] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Hep G2 cells. Antibodies: Affinity purified rabbit anti-HNF1-alpha antibody NBP2-32123 used for IP at 6 ug/mg lysate. HNF1-alpha was also immunoprecipitated by rabbit anti-HNF1-alpha antibodies NBP2-32124, NBP2-32125, NBP2-32126. For blotting immunoprecipitated HNF1-alpha, NBP2-32125 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: HNF1 Antibody [NBP2-32123] - Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- HNF1-alpha used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-NBP2-32123-img0002.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-32126] - Serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- HNF1-alpha used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-Paraffin-NBP2-32126-img0004.jpg "Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-32126] - Serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- HNF1-alpha used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).")
![Immunoprecipitation: HNF1 Antibody [NBP2-32126] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from Hep G2 cells. Antibodies: Affinity purified rabbit anti-HNF1-alpha antibody NBP2-32126used for IP at 6 ug/mg lysate. HNF1-alpha was also immunoprecipitated by rabbit anti-HNF1-alpha antibodies NBP2-32123, NBP2-32124, NBP2-32126. For blotting immunoprecipitated NBP2-32126 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunoprecipitation-NBP2-32126-img0003.jpg)
![Western Blot: HNF1 Antibody [NBP2-32124] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Hep G2 cells. Antibodies: Affinity purified rabbit anti-HNF1-alpha antibody NBP2-32124 used for IP at 6 ug/mg lysate. HNF1-alpha was also immunoprecipitated by rabbit anti-HNF1-alpha antibodies NBP2-32123, NBP2-32125, NBP2-32126. For blotting immunoprecipitated HNF1-alpha, NBP2-32125 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/HNF1-Antibody-Western-Blot-NBP2-32124-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: HNF1 Antibody [NBP2-32124] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Hep G2 cells. Antibodies: Affinity purified rabbit anti-HNF1-alpha antibody NBP2-32124 used for IP at 6 ug/mg lysate. HNF1-alpha was also immunoprecipitated by rabbit anti-HNF1-alpha antibodies NBP2-32123, NBP2-32125, NBP2-32126. For blotting immunoprecipitated HNF1-alpha, NBP2-32125 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: HNF1 Antibody [NBP2-32124] - Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- HNF1-alpha used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-NBP2-32124-img0002.jpg)
![Western Blot: HNF1 Antibody [NBP3-38029] - Western blot analysis of various lysates using HNF1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38029_rabbit-hnf1-pab-101220241233878.jpg "Western Blot: HNF1 Antibody [NBP3-38029] - Western blot analysis of various lysates using HNF1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: HNF1 Overexpression Lysate (Adult Normal) [NBL1-11630] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HNF1.](http://images.novusbio.com/fullsize/HNF1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11630-img0002.jpg "Western Blot: HNF1 Overexpression Lysate (Adult Normal) [NBL1-11630] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HNF1.")
![Immunocytochemistry/Immunofluorescence: HNF1 Antibody [NBP2-98696] - Immunofluorescence staining of HNF1 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum. Then incubated with NBP2-98696 antibody (1:1000)(Figure A), incubated with NBP2-98696 antibody and antigen (Figure B) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/nbp2-98696_rabbit-polyclonal-hnf1-antibody-235202511545681.png "Immunocytochemistry/Immunofluorescence: HNF1 Antibody [NBP2-98696] - Immunofluorescence staining of HNF1 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum. Then incubated with NBP2-98696 antibody (1:1000)(Figure A), incubated with NBP2-98696 antibody and antigen (Figure B) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.")