HNF-4 alpha/NR2A1 Antibody

Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NB100-1783] - Staining of Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: HNF-4 alpha/NR2A1 Antibody [NB100-1783] - (0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Pm, Po, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HNF-4 alpha/NR2A1 Antibody Summary

• Description: Novus Biologicals Goat HNF-4 alpha/NR2A1 Antibody (NB100-1783) is a polyclonal antibody validated for use in IHC, WB, ELISA and ChIP. Anti-HNF-4 alpha/NR2A1 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence RLSKTLVDMDMADY-C corresponding to N-Terminus according to NP_849180.1, NP_000448.3, NP_849181.1.
• Specificity: This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).
• Predicted Species: Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HNF4A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1-0.5 ug/ml
• Application Notes: WB: Approx 55kDa and 48kDa bands observed in lysates of cell line HepG2 (calculated MW of 52.8kDa according to Human NP_000448.3 , 46.6kDa according to Human NP_849181.1). Primary incubation was 1 hour.
• Control:

  Human Tonsil Whole Tissue Lysate (Adult Whole Normal)
  HNF-4 alpha/NR2A1 Overexpression Lysate
  Human Lymph Node Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Primate reactivity reported in scientific literature (PMID18561282).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HNF-4 alpha/NR2A1 Antibody

• FLJ39654
• hepatic nuclear factor 4 alpha
• hepatocyte nuclear factor 4, alpha
• hepatocyte nuclear factor 4-alpha
• HNF4 alpha
• HNF-4 alpha
• HNF4A
• HNF4a7
• HNF-4-alpha
• HNF4alpha10/11/12
• HNF4HNF4a8
• MODY
• MODY1
• NR2A1
• NR2A1HNF4a9
• NR2A21
• Nuclear receptor subfamily 2 group A member 1
• TCF
• TCF14
• TCF-14
• TCF14HNF4alpha
• Transcription factor 14
• Transcription factor HNF-4
• transcription factor-14

Background

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HNF-4 alpha/NR2A1 Antibody (NB100-1783)(6)

Publications using NB100-1783

• Damcott CM, Hoppman N, Ott SH et al. Polymorphisms in both promoters of hepatocyte nuclear factor 4-alpha are associated with type 2 diabetes in the Amish. Diabetes 2004-12-01 [PMID: 15561969]
• Clark A, Saade M, Vemireddy V et al. HNF4? mediated QPRT expression in the kidney facilitates resilience against acute kidney injury bioRxiv 2023-05-25 (ChIP, Mouse)
• Clark AJ, Saade MC, Vemireddy V et al. Hepatocyte nuclear factor 4? mediated quinolinate phosphoribosylltransferase (QPRT) expression in the kidney facilitates resilience against acute kidney injury Kidney international 2023-09-30 [PMID: 37783445]
• Wang SH, Yeh SH, Lin WH, Yeh KH, Yuan Q, Xia NS, Chen DS, Chen PJ. Estrogen Receptor alpha Represses Transcription of HBV Genes via Interaction With Hepatocyte Nuclear Factor 4alpha. Gastroenterology;142(4):989-998. 2012-04-01 [PMID: 22240483] (WB, Human)
• Huang J, Levitsky LL, Rhoads DB et al. Novel P2 promoter-derived HNF4alpha isoforms with different N-terminus generated by alternate exon insertion. Exp Cell Res 2009-04-15 [PMID: 19353766]
• Huang J, Karakucuk V, Levitsky LL et al. Expression of HNF4alpha variants in pancreatic islets and Ins-1 beta cells. Diabetes Metab Res Rev 2008-10-01 [PMID: 18561282] (WB, Primate)

Review for HNF-4 alpha/NR2A1 Antibody (NB100-1783) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Marisa Ferreira ICC Mouse 06/29/2023

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse Liver Tissue
• Species: Mouse
• Lot: P1

Comments

• Comments: ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Bioinformatics

• Gene Symbol: HNF4A
• Entrez:
  • 3172(Human)
• Uniprot:
  • NP_849180.1(Human)