HNF-4 alpha/NR2A1 Antibody (843716) [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. Gels were loaded with 10 µg of cytoplasmic (Cyto) and 5 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HNF-4a/ NR2A1 Monoclonal Antibody (Catalog # MAB4605) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for HNF-4a/NR2A1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HNF-4a/NR2A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human HNF-4a/NR2A1 Monoclonal Antibody (Catalog # MAB4605) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for HNF‑4 alpha /NR2A1 at approximately 62 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human HNF‑4 alpha /NR2A1 Monoclonal Antibody (Catalog # MAB4605). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human HNF-4 alpha/NR2A1 Monoclonal Antibody (Catalog # MAB4605, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

BG01V human embryonic stem cell line differentiated to endodermal cells (StemXVivo Endoderm Kit, Catalog # SC019B) was stained with Mouse Anti-Human HNF-4 alpha/NR2A1 Monoclonal Antibody (Catalog # MAB4605, filled histogram or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: WB, Simple Western, Flow, ICC/IF
• Clone: 843716
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

HNF-4 alpha/NR2A1 Antibody (843716) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human HNF‑4 alpha /NR2A1
  Val130-Ser330
  Accession # P41235
• Specificity: Detects human HNF‑4 alpha /NR2A1 in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNF4A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HNF-4 alpha/NR2A1 Antibody (843716) [Unconjugated]

• FLJ39654
• hepatic nuclear factor 4 alpha
• hepatocyte nuclear factor 4, alpha
• hepatocyte nuclear factor 4-alpha
• HNF4 alpha
• HNF-4 alpha
• HNF4A
• HNF4a7
• HNF-4-alpha
• HNF4alpha10/11/12
• HNF4HNF4a8
• MODY
• MODY1
• NR2A1
• NR2A1HNF4a9
• NR2A21
• Nuclear receptor subfamily 2 group A member 1
• TCF
• TCF14
• TCF-14
• TCF14HNF4alpha
• Transcription factor 14
• Transcription factor HNF-4
• transcription factor-14

Background

HNF-4 alpha is a transcription factor that binds DNA as a homodimer. HNF‑4 alpha is important in liver, kidney, and intestinal development. It has also been intensely studied as one of a variety of genes responsible for diabetes mellitus. HNF-4 alpha has been shown in knock out mice to be essential for the morphogenic and functional differentiation of hepatocytes. HNF-4 alpha is a dominant regulator of epithelial phenotypes able to drive the mesenchymal-to-epithelial transition when expressed in fibroblasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HNF-4 alpha/NR2A1 Antibody (MAB4605)(3)

Publications using MAB4605

• Schnell, J;Miao, Z;Achieng, M;Fausto, CC;Wang, V;Kuyper, F;Thornton, ME;Grubbs, B;Kim, J;Lindström, NO; Stepwise developmental mimicry generates proximal-biased kidney organoids bioRxiv : the preprint server for biology 2024-07-02 [PMID: 39005387] (Immunohistochemistry, Mouse)
• Chen, L;Zhang, L;Jin, G;Liu, Y;Guo, N;Sun, H;Jiang, Y;Zhang, X;He, G;Lv, G;Yang, J;Tu, X;Dong, T;Liu, H;An, J;Si, G;Kang, Z;Li, H;Yi, S;Chen, G;Liu, W;Yang, Y;Ou, J; Synergy of 5-aminolevulinate supplement and CX3CR1 suppression promotes liver regeneration via elevated IGF-1 signaling Cell reports 2023-08-13 [PMID: 37578861] (IHC, Mouse)
• Huang B, Zeng Z, Li H et al. Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids bioRxiv 2023-05-26 [PMID: 37293038]

Review for HNF-4 alpha/NR2A1 Antibody (MAB4605) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Pig.

reviewed by: Verified Customer ICC Pig 12/05/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: Pig trabecular meshwork cells
• Species: Pig

Bioinformatics

• Gene Symbol: HNF4A
• Uniprot:
  • P41235()