HNF-4 alpha/NR2A1 Antibody (843716) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: Flow
• Clone: 843716
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

HNF-4 alpha/NR2A1 Antibody (843716) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human HNF‑4 alpha /NR2A1
  Val130-Ser330
  Accession # P41235
• Specificity: Detects human HNF‑4 alpha /NR2A1 in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HNF-4 alpha/NR2A1 Antibody (843716) [Alexa Fluor® 647]

• FLJ39654
• hepatic nuclear factor 4 alpha
• hepatocyte nuclear factor 4, alpha
• hepatocyte nuclear factor 4-alpha
• HNF4 alpha
• HNF-4 alpha
• HNF4A
• HNF4a7
• HNF-4-alpha
• HNF4alpha10/11/12
• HNF4HNF4a8
• MODY
• MODY1
• NR2A1
• NR2A1HNF4a9
• NR2A21
• Nuclear receptor subfamily 2 group A member 1
• TCF
• TCF14
• TCF-14
• TCF14HNF4alpha
• Transcription factor 14
• Transcription factor HNF-4
• transcription factor-14

Background

HNF-4 alpha is a transcription factor that binds DNA as a homodimer. HNF‑4 alpha is important in liver, kidney, and intestinal development. It has also been intensely studied as one of a variety of genes responsible for diabetes mellitus. HNF-4 alpha has been shown in knock out mice to be essential for the morphogenic and functional differentiation of hepatocytes. HNF-4 alpha is a dominant regulator of epithelial phenotypes able to drive the mesenchymal-to-epithelial transition when expressed in fibroblasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HNF-4 alpha/NR2A1 Antibody (IC4605R)(2)

Publications using IC4605R

• Lyra-Leite, DM;Copley, RR;Freeman, PP;Pongpamorn, P;Shah, D;McKenna, DE;Lenny, B;Pinheiro, EA;Weddle, CJ;Gharib, M;Javed, H;Fonoudi, H;Sapkota, Y;Burridge, PW; Nutritional requirements of human induced pluripotent stem cells Stem cell reports 2023-06-13 [PMID: 37315525] (Flow Cytometry, Human)
• G Pettinato, MF Coughlan, X Zhang, L Chen, U Khan, M Glyavina, CJ Sheil, PK Upputuri, YN Zakharov, E Vitkin, AB D'Assoro, RA Fisher, I Itzkan, L Zhang, L Qiu, LT Perelman Spectroscopic label-free microscopy of changes in live cell chromatin and biochemical composition in transplantable organoids Science Advances, 2021-08-18;7(34):. 2021-08-18 [PMID: 34407934] (Flow Cytometry, Human)

Bioinformatics

• Uniprot:
  • P41235()