HNF-4 alpha/NR2A1 Antibody (10A7) - BSA Free

Immunohistochemistry: HNF-4 alpha/NR2A1 Antibody (10A7) [NBP3-26222] - Image of HNF-4 alpha/NR2A1 Antibody (10A7) diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.75% DAB.

Western Blot: HNF-4 alpha/NR2A1 Antibody (10A7) [NBP3-26222] - Positive Western Blot detected in: HL-60 whole cell lysate, Rat Liver tissue lysate. All lanes: HNF-4 alpha/NR2A1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52 kDa Observed band size: 52 kDa

Immunocytochemistry/Immunofluorescence: HNF-4 alpha/NR2A1 Antibody (10A7) [NBP3-26222] - Staining of MCF-7 with HNF-4 alpha/NR2A1 Antibody (10A7) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 526-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: HNF-4 alpha/NR2A1 Antibody (10A7) [NBP3-26222] - Overlay Peak curve showing HepG2 cells stained with HNF-4 alpha/NR2A1 Antibody (10A7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 10A7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HNF-4 alpha/NR2A1 Antibody (10A7) - BSA Free Summary

• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human HNF-4 alpha/NR2A1 [UniProt P41235]
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HNF4A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HNF-4 alpha/NR2A1 Antibody (10A7) - BSA Free

• FLJ39654
• hepatic nuclear factor 4 alpha
• hepatocyte nuclear factor 4, alpha
• hepatocyte nuclear factor 4-alpha
• HNF4 alpha
• HNF-4 alpha
• HNF4A
• HNF4a7
• HNF-4-alpha
• HNF4alpha10/11/12
• HNF4HNF4a8
• MODY
• MODY1
• NR2A1
• NR2A1HNF4a9
• NR2A21
• Nuclear receptor subfamily 2 group A member 1
• TCF
• TCF14
• TCF-14
• TCF14HNF4alpha
• Transcription factor 14
• Transcription factor HNF-4
• transcription factor-14

Background

HNF4 alpha is encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNF4A