HMGB1/HMG-1 Antibody

Images

 
Western Blot: HMGB1/HMG-1 Antibody [NBP2-27169] - Analysis using HMGB1 antibody. Mouse NIH 3T3 cell lysate was probed with HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate ...read more
Simple Western: HMGB1/HMG-1 Antibody [NBP2-27169] - Simple Western lane view shows a specific band for HMGB1 in 0.2 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, ChSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HMGB1/HMG-1 Antibody Summary

Immunogen
A portion of amino acids 25-94 of human HMGB1 was used as the immunogen.
Predicted Species
Chicken (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HMGB1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 1:200
  • Western Blot 5-10ug/ml
Application Notes
Minimal staining in IHC and ELISPOT indicates that this antibody preferentially detects the reduced form of HMGB1.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reactivity Notes

The HMGB1 amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, and monkey. This antibody recognizes both A box domain (amino acids 9-79) and full length HMGB1 protein.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml sterile PBS
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for HMGB1/HMG-1 Antibody

  • Amphoterin
  • high mobility group box 1
  • High mobility group protein 1
  • high mobility group protein B1
  • high-mobility group (nonhistone chromosomal) protein 1
  • high-mobility group box 1
  • HMG1
  • HMG-1
  • HMG1DKFZp686A04236
  • HMG3
  • HMGB1
  • SBP-1
  • Sulfoglucuronyl carbohydrate binding protein

Background

HMGB1 (high mobility group protein B1) belongs to the HMGB family and contains two HMG box DNA-binding domains. It is a highly conserved, ubiquitous protein present in the nuclei and cytoplasm of nearly all cell types, and is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). It is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for HMGB1/HMG-1 Antibody (NBP2-27169). (Showing 1 - 1 of 1 FAQ).

  1. Are there any referencing publications for this product?
    • Unfortunately, we do not have any publications referenced for this antibody. The only available validation information for WB is what is on the website/datasheet. 

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HMGB1