HMGB1/HMG-1 Antibody - Azide and BSA Free

Western Blot: HMGB1/HMG-1 Antibody [NBP2-27169] - Analysis using HMGB1 antibody. Mouse NIH 3T3 cell lysate was probed with HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Simple Western: HMGB1/HMG-1 Antibody [NBP2-27169] - Simple Western lane view shows a specific band for HMGB1 in 0.2 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Ch
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

HMGB1/HMG-1 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit HMGB1/HMG-1 Antibody - Azide and BSA Free (NBP2-27169) is a polyclonal antibody validated for use in WB and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 25-94 of human HMGB1 was used as the immunogen.
• Predicted Species: Chicken (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HMGB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:200
  • Western Blot 5-10ug/ml
• Application Notes: Minimal staining in IHC and ELISPOT indicates that this antibody preferentially detects the reduced form of HMGB1.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Jurkat lysate 0.2 mg/mL, separated by Size, antibody dilution of 1:200, apparent MW was 36 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reactivity Notes

The HMGB1 amino acid sequence used as immunogen is 100% homologous in rat, and monkey.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for HMGB1/HMG-1 Antibody - Azide and BSA Free

• Amphoterin
• high mobility group box 1
• High mobility group protein 1
• high mobility group protein B1
• high-mobility group (nonhistone chromosomal) protein 1
• high-mobility group box 1
• HMG1
• HMG-1
• HMG1DKFZp686A04236
• HMG3
• HMGB1
• SBP-1
• Sulfoglucuronyl carbohydrate binding protein

Background

HMGB1 (high mobility group protein B1) belongs to the HMGB family and contains two HMG box DNA-binding domains. It is a highly conserved, ubiquitous protein present in the nuclei and cytoplasm of nearly all cell types, and is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). It is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for HMGB1/HMG-1 Antibody (NBP2-27169). (Showing 1 - 1 of 1 FAQ).

1. Are there any referencing publications for this product?
   • Unfortunately, we do not have any publications referenced for this antibody. The only available validation information for WB is what is on the website/datasheet. 

Bioinformatics

• Gene Symbol: HMGB1