HMGA2 Antibody (763)

Images

 
Western Blot: HMGA2 Antibody (763) [NBP2-43640] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [763] diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: HMGA2 Antibody (763) [NBP2-43640] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGA2 protein stained by HMGA2 antibody [763] diluted at 1:200. Red: ...read more
Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640] - Mouse brain. HMGA2 stained by HMGA2 antibody [763] (GTX629478) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: HMGA2 Antibody (763) [NBP2-43640] - Analysis of A. 30 ug HepG2 whole cell lysate/extract 15 % SDS-PAGE HMGA2 antibody [763] dilution: 1:1000
Western Blot: HMGA2 Antibody (763) [NBP2-43640] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [763] diluted at 1:1000.
Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640] - C2C12. HMGA2 antibody [763] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640] - RT2 xenograft. HMGA2 antibody [763] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640] - SkHep1 xenograft. HMGA2 antibody [763] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640] - Rat heart. HMGA2 stained by HMGA2 antibody [763] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunoprecipitation: HMGA2 Antibody (763) [NBP2-43640] - Analysis of HMGA2 protein from HepG2 nuclear extracts using 5 ug of HMGA2 antibody [763]. Western blot analysis was performed using HMGA2 antibody [763]. EasyBlot ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
763
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HMGA2 Antibody (763) Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HMGA2. The exact sequence is proprietary.
Predicted Species
Chicken (98%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HMGA2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-43640 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Zebrafish (85%), Xenopus laevis (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for HMGA2 Antibody (763)

  • BABL
  • BABLHMGI-C
  • high mobility group AT-hook 2
  • High mobility group AT-hook protein 2
  • high mobility group protein HMGI-C
  • high-mobility group (nonhistone chromosomal) protein isoform I-C
  • High-mobility group protein HMGI-C
  • HMGA2
  • HMGIC
  • HMGICSTQTL9
  • LIPO

Background

HMGA2 encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HMGA2 Antibody (NBP2-43640)(2)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(1)
WB
(1)
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Filter By Species
Human
(1)
Rat
(1)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HMGA2