HMGA2 Antibody (763) - Azide and BSA Free

Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640] - Mouse brain. HMGA2 stained by HMGA2 antibody [763] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: HMGA2 Antibody (763) [NBP2-43640] - Analysis of A. 30 ug HepG2 whole cell lysate/extract 15 % SDS-PAGE HMGA2 antibody [763] dilution: 1:1000

Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640] - C2C12. HMGA2 antibody [763] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640] - RT2 xenograft. HMGA2 antibody [763] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640] - SkHep1 xenograft. HMGA2 antibody [763] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640] - Rat heart. HMGA2 stained by HMGA2 antibody [763] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation: HMGA2 Antibody (763) [NBP2-43640] - Analysis of HMGA2 protein from HepG2 nuclear extracts using 5 ug of HMGA2 antibody [763]. Western blot analysis was performed using HMGA2 antibody [763]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Western Blot: HMGA2 Antibody (763) [NBP2-43640] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] (NBP2-43640) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] (NBP2-43640) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

HMGA2 antibody [GT763] detects HMGA2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGA2 protein stained by HMGA2 antibody [GT763] (NBP2-43640) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB, ICC/IF, IHC, IP
• Clone: 763
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HMGA2 Antibody (763) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HMGA2 Antibody (763) - Azide and BSA Free (NBP2-43640) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-HMGA2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HMGA2. The exact sequence is proprietary.
• Predicted Species: Chicken (98%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMGA2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (85%), Xenopus laevis (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for HMGA2 Antibody (763) - Azide and BSA Free

• BABL
• BABLHMGI-C
• high mobility group AT-hook 2
• High mobility group AT-hook protein 2
• high mobility group protein HMGI-C
• high-mobility group (nonhistone chromosomal) protein isoform I-C
• High-mobility group protein HMGI-C
• HMGA2
• HMGIC
• HMGICSTQTL9
• LIPO

Background

HMGA2 encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HMGA2 Antibody (NBP2-43640)(2)

Publications using NBP2-43640

• Jiao W, Hao J, Xie y et al. EZH2 mitigates the cardioprotective effects of mesenchymal stem cell-secreted exosomes against infarction via HMGA2-mediated PI3K/AKT signaling BMC cardiovascular disorders 2022-03-09 [PMID: 35264108] (WB, Rat)
• Kondo Y, Hirabayashi K, Carreras J et al. The significance of tyrosine kinase receptor B and brain-derived neurotrophic factor expression in salivary duct carcinoma Biomed Mater 2020-11-26 [PMID: 33248386] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: HMGA2