Human HMG-CoA Reductase/HMGCR ELISA Kit (Colorimetric)

Standard Curve Reference.

Samples were spiked with high concentrations of Human HMG-CoA Reductase/HMGCR, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.63 - 40 ng/mL
• Sensitivity: 0.38 ng/mL

Human HMG-CoA Reductase/HMGCR ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 3 h 30 min
• Specificity: This kit recognizes Human HMG-CoA Reductase/HMGCR in samples. No significant cross-reactivity or interference between Human HMG-CoA Reductase/HMGCR and analogues was observed
• Standard Curve Range: 0.63 - 40 ng/mL
• Sensitivity: 0.38 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10
• Intra-Assay: CV% < 10
• Spike Recovery: 80 - 120%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HMGCR

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human HMG-CoA Reductase/HMGCR ELISA Kit (Colorimetric)

• 3-hydroxy-3-methylglutaryl CoA reductase (NADPH)
• 3-hydroxy-3-methylglutaryl-CoA reductase
• 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
• EC 1.1.1
• EC 1.1.1.34
• HMG-CoA Reductase
• HMGCR
• Hydroxymethylglutaryl-CoA Reductase
• LDLCQ3

Background

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for HMG-CoA Reductase/HMGCR ELISA Kit (NBP3-49780). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: HMGCR