HMG-CoA Reductase/HMGCR Antibody (CL0259) - Azide and BSA Free

Analysis in human liver and skeletal muscle tissues using NBP3-43943 antibody. Corresponding HMG-CoA Reductase/HMGCR RNA-seq data are presented for the same tissues.

Staining of human breast cancer shows moderate cytoplasmic positivity in tumor cells.

Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Staining of human liver cancer shows strong cytoplasmic positivity in tumor cells.

Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HMG-CoA Reductase/HMGCR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Staining of human uterine cervix shows moderate cytoplasmic positivity in squamous epithelial cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0259
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

HMG-CoA Reductase/HMGCR Antibody (CL0259) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse HMG-CoA Reductase/HMGCR Antibody (CL0259) - Azide and BSA Free (NBP2-61616) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P04035, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMGCR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 1 ug/ml
• Application Notes: IHC-Paraffin HIER pH6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for HMG-CoA Reductase/HMGCR Antibody (CL0259) - Azide and BSA Free

• 3-hydroxy-3-methylglutaryl CoA reductase (NADPH)
• 3-hydroxy-3-methylglutaryl-CoA reductase
• 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
• EC 1.1.1
• EC 1.1.1.34
• HMG-CoA Reductase
• HMGCR
• Hydroxymethylglutaryl-CoA Reductase
• LDLCQ3

Background

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMGCR