Histone H3 [ac Lys9, ac Lys14] Antibody


Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - WB analysis of H3K9ac/K14ac in C. elegans embryo lysate.
Immunocytochemistry: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - Immunofluorescence: [NB21-1081] - Histone H3.2 K9,K14ac antibody was tested at 1:50 in HeLa cells with FITC. Nuclei and actin were ...read more
Western Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - WB analysis of H3 K9ac/K14ac in NIH/3T3 histone preps.
Dot Blot: Histone H3 [ac Lys9, ac Lys14] Antibody [NB21-1081] - Dot blot analysis of H3 K36ac in picomoles of peptide.

Product Details

Reactivity Hu, Mu, CeSpecies Glossary
Applications WB, ChIP, DB, ICC/IF
0.63 mg/ml

Histone H3 [ac Lys9, ac Lys14] Antibody Summary

Synthetic acetylated peptide surrounding Lysines 9 and 14 of human Histone H3.2 [Swiss Prot Q71DI3].
ac Lys9, ac Lys14
Nucleus. Chromosome.
Predicted Species
Rat (100%), Chicken (100%), Xenopus (100%), Drosophila (100%), Plant (100%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.05% Sodium Azide
0.63 mg/ml
Immunogen affinity purified


  • Western Blot 1:500
  • Chromatin Immunoprecipitation 2-5 ug per million cells
  • Dot Blot 0.1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:200
Application Notes
This H3K9ac/K14ac antibody is useful for ChIP, ICC/IF, Dot Blot, and Western Blot where a band is seen ~15 kDa in NIH 3T3 histone prep and C. elegans embryo lysate.
Read Publication using
NB21-1081 in the following applications:

Reactivity Notes

Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.


Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.

Alternate Names for Histone H3 [ac Lys9, ac Lys14] Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • H3F3A
  • histone H3.3
  • MGC87782
  • MGC87783


Acetylation at K9 and K14 of histone H3 is known to be an epigenetic modification associated with active transcription. Under cellular stress conditions, H3K9ac/K14ac levels increase, probably due to the inactivation of the HDA6 histone deacetylase. Particularly, during infections with herpes simplex virus, the acute phase of the infection is demarcated by increased presence of H3K9ac/K14ac associated with transcriptionally active euchromatin. The virus seems to take advantage of the highly transcribed regions of euchromatin for viral replication, increasing the lytic infection.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H3 Antibody (NB21-1081)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: ChIP, ICC/IF.

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Product General Protocols

View specific protocols for Histone H3 Antibody (NB21-1081): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NB21-1081) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB21-1081AF405
Alexa Fluor (R) 488 Labeled NB21-1081AF488
Alexa Fluor (R) 647 Labeled NB21-1081AF647
Alexa Fluor (R) 700 Labeled NB21-1081AF700
Biotin Labeled NB21-1081B
DyLight 405 Labeled NB21-1081V
DyLight 488 Labeled NB21-1081G
DyLight 550 Labeled NB21-1081R
DyLight 650 Labeled NB21-1081C
FITC Labeled NB21-1081F
HRP Labeled NB21-1081H

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The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Understanding the Reasons for Histone H3 K4 Trimethylation (H3K4Me3)
Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that...  Read full blog post.

The 'epi-genie' is Out of the Bottle: Functional Histone 3 Variants in Human Disease
Discovery of histone variants using highly specific antibodies has led to the emerging notion that alterations in histone modifications and further changes in chromatin structure are induced by exchange of histone variants. Covalent histone modificati...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol HIST2H3C

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