Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - WB analysis of H3K9me2 in C. elegans embryo lysate.
Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Histone H3.2 K9me2 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1072 and 20ul of magnetic IgG beads per ...read more
Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Western blot analysis of Histone H3K9me2 on HeLa histone preps.
Dot Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Dot blot analysis of H3K9me2 in picomoles of peptide.
Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.
Alternate Names for Histone H3 [Dimethyl Lys9] Antibody
H3 histone, family 3A
Dimethylated histone H3 at lysine 9 is associated with several inflammatory diseases. Usually, the histone modification is implicated through a one-off association with critical disease related genes. For example, in Alzheimers disesase, amyloid beta-peptide accumulation is caused by insufficient neprilysin, which is in turn caused by increased concentrations of H3K9me2 in the neprilysin promoter following exposure to hypoxia. In osteoarthritis, increased H3K9me2 at the NFAT1 promoter represses the expression of NFAT, resulting in overexpression of proinflammatory cytokines. PHF2 and ARID5B are important in regulating the methylation of H3 at K9, and thus chromatin reorganization and gene transcription.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The role of DNMT3A in development Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence. Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation. Gene silencing in DNA ... Read full blog post.
Histone H3 Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me... Read full blog post.
EZH2: Epigenetic Regulation Made Easy! Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i... Read full blog post.