Histone H3 [Dimethyl Lys9] Antibody

Images

 
Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - WB analysis of H3K9me2 in C. elegans embryo lysate.
Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Histone H3.2 K9me2 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1072 and 20ul of magnetic IgG beads per ...read more
Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Western blot analysis of Histone H3K9me2 on HeLa histone preps.
Dot Blot: Histone H3 [Dimethyl Lys9] Antibody [NB21-1072] - Dot blot analysis of H3K9me2 in picomoles of peptide.

Product Details

Summary
Reactivity Hu, Mu, Ce, Chicken, Drosophila, Plant, Rat, XenopusSpecies Glossary
Applications WB, ChIP, DB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Concentration
1.0 mg/ml

Histone H3 [Dimethyl Lys9] Antibody Summary

Immunogen
Synthetic dimethylated peptide surrounding Lysine 9 of human Histone H3.2 [Swiss Prot Q71DI3].
Modification
Dimethyl Lys9
Localization
Nucleus. Chromosome.
Predicted Species
Rat (100%), Chicken (100%), Drosophila (100%), Xenopus (100%), Plant (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
HIST2H3C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Chromatin Immunoprecipitation 2-5 ug per million cells
  • Dot Blot 0.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50
Application Notes
This Histone H3 K9me2 antibody is useful for ChIP, ICC/IF, Dot Blot, and Western Blot where a band is seen ~15 kDa in HeLa histone preps and C. elegans embryo lysate.
Publications
Read Publication using
NB21-1072 in the following applications:

Reactivity Notes

Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.

Notes

Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.

Alternate Names for Histone H3 [Dimethyl Lys9] Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • H3F3A
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783

Background

Dimethylated histone H3 at lysine 9 is associated with several inflammatory diseases. Usually, the histone modification is implicated through a one-off association with critical disease related genes. For example, in Alzheimers disesase, amyloid beta-peptide accumulation is caused by insufficient neprilysin, which is in turn caused by increased concentrations of H3K9me2 in the neprilysin promoter following exposure to hypoxia. In osteoarthritis, increased H3K9me2 at the NFAT1 promoter represses the expression of NFAT, resulting in overexpression of proinflammatory cytokines. PHF2 and ARID5B are important in regulating the methylation of H3 at K9, and thus chromatin reorganization and gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H3 Antibody (NB21-1072)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ChIP.


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ChIP
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Product General Protocols

View specific protocols for Histone H3 Antibody (NB21-1072): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NB21-1072) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB21-1072AF405
Alexa Fluor 488 Labeled NB21-1072AF488
Alexa Fluor 647 Labeled NB21-1072AF647
Alexa Fluor 700 Labeled NB21-1072AF700
Biotin Labeled NB21-1072B
DyLight 405 Labeled NB21-1072V
DyLight 488 Labeled NB21-1072G
DyLight 550 Labeled NB21-1072R
DyLight 650 Labeled NB21-1072C
HRP Labeled NB21-1072H

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Research Areas for Histone H3 Antibody (NB21-1072)

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Blogs on Histone H3.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Understanding the Reasons for Histone H3 K4 Trimethylation (H3K4Me3)
Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that...  Read full blog post.

The 'epi-genie' is Out of the Bottle: Functional Histone 3 Variants in Human Disease
Discovery of histone variants using highly specific antibodies has led to the emerging notion that alterations in histone modifications and further changes in chromatin structure are induced by exchange of histone variants. Covalent histone modificati...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol HIST2H3C
Entrez
Uniprot

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