Histone H3 [p Thr6, Trimethyl Lys4] Antibody

Images

 
Western Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - WB analysis of H3K4Me3/pT6 in C. elegans embryo lysate.
Immunocytochemistry/ Immunofluorescence: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - Immunofluorescence: [NB21-1039] - Histone H3.2 K4me3 pT6 antibody was tested in HeLa cells with FITC (green). Nuclei ...read more
Chromatin Immunoprecipitation: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - 2 ug of NB21-1039 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control, DNA was measured by qRT-PCR ...read more
Western Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - WB detection of H3K4me3/pT6 in HeLa histone preps.
Dot Blot: Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039] - Dot blot analysis of H3K4me3/pT6 with the peptides stated above in picomoles of peptide.
Histone H3 [p Thr6, Trimethyl Lys4] Antibody [NB21-1039]

Product Details

Summary
Reactivity Hu, Mu, Ce, Rt, Ch, Dr, Pl, XpSpecies Glossary
Applications WB, ChIP, DB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.68 mg/ml

Histone H3 [p Thr6, Trimethyl Lys4] Antibody Summary

Immunogen
Synthetic trimethylated/phosphorylated peptides surrounding Lysine 4 and Threonine 6 of human Histone H3.2 [Swiss Prot Q71DI3].
Modification
Trimethyl Lys4, p Thr6
Localization
Nucleus. Chromosome.
Predicted Species
Rat (100%), Plant (100%), Xenopus (100%), Drosophila (100%), Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST2H3C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Chromatin Immunoprecipitation 2-5 ug per million cells
  • Dot Blot 0.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50
Application Notes
This Histone H3 trimethyl K4/phospho T6 antibody is useful for ChIP, Immunocytochemistry/Immunofluorescence, Dot Blot, and Western Blot where a band is seen ~15 kDa in HeLa histone prep and C. elegans embryo lysate.
Positive Control
GAPDH primer (NBP1-71650)
RPL30 primer (NBP1-71651)
MYOD1 primer (NBP1-71652)
AFM primer (NBP1-71653)
alpha Satellite repeat primer (NBP1-71654)
Satellite2 repeat primer (NBP1-71655)
Control Peptide
Histone H3 Peptide (NB21-1039PEP)

Reactivity Notes

Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.68 mg/ml
Purity
Immunogen affinity purified

Notes

Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.

Alternate Names for Histone H3 [p Thr6, Trimethyl Lys4] Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • H3F3A
  • H3K4Me3
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783

Background

Phosphorylation at T6 of methylated H3K4 prevents LSD1 from demethylating histone H3. Androgen receptor activated gene expression depends upon removal of methyl groups from H3K4, in cooperation with the Jumonji protein JMJD2C. However, when T6 is phosphorylated, there is a physical obstruction in the way of demethylation, and thus gene expression is repressed. The PHD finger of H3K4 seems to be an effector of histone modification, which can cause dysfunction in cellular fate regulation. Interestingly, the abundance of phosphorylation of this modified histone is a probable biomarker for the detection and the prognosis of certain cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ChIP, DB, ICC/IF

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Product General Protocols

View specific protocols for Histone H3 Antibody (NB21-1039): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NB21-1039) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB21-1039AF405
Alexa Fluor 488 Labeled NB21-1039AF488
Alexa Fluor 647 Labeled NB21-1039AF647
Alexa Fluor 700 Labeled NB21-1039AF700
Biotin Labeled NB21-1039B
DyLight 405 Labeled NB21-1039V
DyLight 488 Labeled NB21-1039G
DyLight 550 Labeled NB21-1039R
DyLight 650 Labeled NB21-1039C
FITC Labeled NB21-1039F
HRP Labeled NB21-1039H

Additional Array Products

Bioinformatics Tool for Histone H3 Antibody (NB21-1039)

Discover related pathways, diseases and genes to Histone H3 Antibody (NB21-1039). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Histone H3. Showing 1-10 of 490 blog posts - Show all blog posts.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

Showing 1-10 of 490 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol HIST2H3C
Entrez
Uniprot