Histone H3 [Dimethyl Lys4] Antibody


Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - H3K4Me2 immunoblot in Jurkat cells. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Histone H3 was tested at 1:100 in Hela cells against Dylight 488 (Green). Nuclei were illuminated blue with DAPI.
Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1022 and 20ul of magnetic IgG beads per ...read more
Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Western blot analysis of H3 K4me2 in HeLa histone preps at 1:1000.
Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - WB analysis of H3 K4me2 in C. elegans embryo lysate.
Dot Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Dot blot analysis of H3 K4me2 using the peptides stated above in 25, 10, 3 and 1 picomoles of peptide.
Histone H3 [Dimethyl Lys4] Antibody [NB21-1022]

Product Details

Reactivity Hu, Mu, Ce, Rt, Ch, Dr, Pl, XpSpecies Glossary
Applications WB, ChIP, DB, ICC/IF
1.16 mg/ml

Histone H3 [Dimethyl Lys4] Antibody Summary

Synthetic dimethylated peptide surrounding Lysine 4 of human Histone H3.2 [Swiss Prot Q71DI3].
Dimethyl Lys4
Nucleus. Chromosome.
Predicted Species
Rat (100%), Chicken (100%), Xenopus (100%), Drosophila (100%), Plant (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500
  • Chromatin Immunoprecipitation 2-5 ug per million cells
  • Dot Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50
Application Notes
This Histone H3 K4me2 antibody is useful for ChIP, Dot Blot, Immunocytochemistry/Immunofluorescence, and Western Blot where a band is seen ~15kDa in HeLa histone preps and C. elegans embryo lysate.
Positive Control
GAPDH primer (NBP1-71650)
RPL30 primer (NBP1-71651)
MYOD1 primer (NBP1-71652)
AFM primer (NBP1-71653)
alpha Satellite repeat primer (NBP1-71654)
Satellite2 repeat primer (NBP1-71655)
Control Peptide
Histone H3 Peptide (NB21-1022PEP)
Reviewed Applications
Read 1 Review rated 5
NB21-1022 in the following applications:

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NB21-1022 in the following applications:

Reactivity Notes

Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
1.16 mg/ml
Immunogen affinity purified


Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.

Alternate Names for Histone H3 [Dimethyl Lys4] Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • H3F3A
  • H3K4Me2
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783


H3K4me2 is a modification thought to have a role in transcriptional memory. In CD4+ T lymphocytes, H3K4Me2 is present within gene bodies regulating cellular function, but not in those of housekeeping genes, which indicates that the modification has a role in refining the tissue-specificity of expressed genes (PMID: 20841431). This type of cellular identity targeting is also noted in work with human and mouse spermatozoa (PMID: 20473313); the H3K4Me2 modification marks genes that are relevant in spermatogenesis. Most effects of H3K4Me2 seem to be attributed to its transcriptional activation; however, recent work also indicates that it may also play an RNA-dependent regressive role, related to the GAL-1 promoter (PMID: 19407817).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H3 Antibody (NB21-1022)(1)

We have publications tested in 1 confirmed species: C. elegans.

We have publications tested in 1 application: ICC/IF.

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C. elegans
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Review for Histone H3 Antibody (NB21-1022) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB21-1022:
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Images Ratings Applications Species Date Details
Western Blot Histone H3 NB21-1022
reviewed by:
WB Human 09/19/2014


ApplicationWestern Blot
Sample TestedCell lysate from Jurkat cells grown in RPMI medium


Blocking DetailsNon-fat dry milk (5%) in PBS

Primary Anitbody

Dilution Ratio1:2000 in PBS at RT for 3 hours

Secondary Antibody

Secondary DescriptionAnti rabbit IgG (HRP-conjugated)
Secondary Manufacturer Cat#ab6721 (Abcam)
Secondary Concentration1:10,000


Detection NotesBio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 10sec

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NB21-1022). (Showing 1 - 1 of 1 FAQ).

  1. The description says that this antibody react with C. elegans. Have it been tested in C elegans or is it just a prediction?
    • On our product page for our Histone H3 antibody with catalogue number NB21-1022, the western blot data was generated from C.elegans embryo lysate. This is the only C.elegans data that we currently have for this antibody, but if you would like to add your own data in the form of a product review you would be eligible for points which can be redeemed against a future purchase from Novus, or against an Amazon gift card. View more information for reviews.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB21-1022AF405
Alexa Fluor 488 Labeled NB21-1022AF488
Alexa Fluor 647 Labeled NB21-1022AF647
Alexa Fluor 700 Labeled NB21-1022AF700
Biotin Labeled NB21-1022B
DyLight 405 Labeled NB21-1022V
DyLight 488 Labeled NB21-1022G
DyLight 550 Labeled NB21-1022R
DyLight 650 Labeled NB21-1022C
FITC Labeled NB21-1022F
HRP Labeled NB21-1022H

Additional Array Products

Bioinformatics Tool for Histone H3 Antibody (NB21-1022)

Discover related pathways, diseases and genes to Histone H3 Antibody (NB21-1022). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Histone H3.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Understanding the Reasons for Histone H3 K4 Trimethylation (H3K4Me3)
Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that...  Read full blog post.

The 'epi-genie' is Out of the Bottle: Functional Histone 3 Variants in Human Disease
Discovery of histone variants using highly specific antibodies has led to the emerging notion that alterations in histone modifications and further changes in chromatin structure are induced by exchange of histone variants. Covalent histone modificati...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Recent Reviews


Application: WB
Species: Human


Gene Symbol HIST2H3C