Western Blot: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - WB analysis of Histone H3 [Asym-dimethyl Arg2] in C. elegans embryo lysate.
Immunocytochemistry/ Immunofluorescence: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Histone H3 [Asym-dimethyl Arg2] antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with ...read more
Dot Blot: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Dot blot analysis of Histone H3 [Asym-dimethyl Arg2] in picomoles of peptide.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Western Blot 1:500
Dot Blot 1:1000
This Histone H3 [Asym-dimethyl Arg2] antibodiy is useful for Immunocytochemistry/Immunofluorescence, Dot Blot, and Western Blot where a band is seen ~15 kDa in C. elegans embryo lysate. In ICC/IF, nuclear staining was observed in HeLa cells.
Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.
Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.
Alternate Names for Histone H3 [Asym-dimethyl Arg2] Antibody
H3 histone, family 3A
When Set1 attempts to methylate H3K4, epigenetic silencing of downstream genes is mediated by dimethylation of histone H3 at Arg2. This modification is typically found in heterochromatin and inactive genes, and is not found when trimethylation of Arg2 is observed. Conversion from mono-, di- and tri-methyl forms of H3R2 is mediated through the activity of the arginine methyltransferase PRMT6. H3K4 cannot be methylated when the asymmetric H3R2me2 mark is present. In active promoters, this modification is actively eliminated. H3R2me2a prevents WDR5 recognition, MLL methyltransferase recruitment, and H3K4 methylation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Histone H3 Antibody (NB21-1002). (Showing 1 - 1 of 1 FAQs).
I received H3R2me2a antibody from your company. I would like to use your antibody for western blotting. May I know the best blocking reagents (5% milk or 5%BSA) for this antibody?
When we generated our Western blot data with NB21-1002 we used a 4-15% gradient gel, and ran this in Tris-glycine buffer for one hour at 100V. The proteins were transferred to a 0.22uM nitrocellulose membrane, and this was blocked for one hour at room temperature with 3% BSA in TBS-T (1x TBS, 0.1% Tween-20). NB21-1002 was diluted to 1:1000 in blocking buffer and incubated at 4C overnight. The secondary antibody was incubated for one hour at room temperature.
The role of DNMT3A in development Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence. Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation. Gene silencing in DNA ... Read full blog post.
Histone H3 Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me... Read full blog post.
EZH2: Epigenetic Regulation Made Easy! Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i... Read full blog post.