Histone H3 [Asym-dimethyl Arg2] Antibody


Western Blot: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - WB analysis of Histone H3 [Asym-dimethyl Arg2] in C. elegans embryo lysate.
Immunocytochemistry/ Immunofluorescence: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Histone H3 [Asym-dimethyl Arg2] antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with ...read more
Dot Blot: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Dot blot analysis of Histone H3 [Asym-dimethyl Arg2] in picomoles of peptide.
Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002]

Product Details

Reactivity Hu, Mu, Ce, Rt, Ch, Dr, Pl, XpSpecies Glossary
Applications WB, ChIP, DB, ICC/IF
0.71 mg/ml

Histone H3 [Asym-dimethyl Arg2] Antibody Summary

Synthetic dimethylated peptide surrounding Arginine 2 of human Histone H3 [Asym-dimethyl Arg2] [Swiss Prot Q71DI3].
Asym-dimethyl Arg2
Nucleus. Chromosome.
Predicted Species
Rat (100%), Xenopus (100%), Drosophila (100%), Plant (100%), Chicken (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500
  • Chromatin Immunoprecipitation
  • Dot Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
Application Notes
This Histone H3 [Asym-dimethyl Arg2] antibodiy is useful for Immunocytochemistry/Immunofluorescence, Dot Blot, and Western Blot where a band is seen ~15 kDa in C. elegans embryo lysate. In ICC/IF, nuclear staining was observed in HeLa cells.
Positive Control
GAPDH primer (NBP1-71650)
RPL30 primer (NBP1-71651)
MYOD1 primer (NBP1-71652)
AFM primer (NBP1-71653)
alpha Satellite repeat primer (NBP1-71654)
Satellite2 repeat primer (NBP1-71655)
Control Peptide
Histone H3 Peptide (NB21-1002PEP)
Read Publications using
NB21-1002 in the following applications:

  • 1 publication
  • WB
    2 publications

Reactivity Notes

Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
0.71 mg/ml
Immunogen affinity purified


Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.

Alternate Names for Histone H3 [Asym-dimethyl Arg2] Antibody

  • H3 histone, family 3A
  • H3.3AH3F3H3F3B
  • H3.3B
  • H3F3A
  • H3R2Me2a
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783


When Set1 attempts to methylate H3K4, epigenetic silencing of downstream genes is mediated by dimethylation of histone H3 at Arg2. This modification is typically found in heterochromatin and inactive genes, and is not found when trimethylation of Arg2 is observed. Conversion from mono-, di- and tri-methyl forms of H3R2 is mediated through the activity of the arginine methyltransferase PRMT6. H3K4 cannot be methylated when the asymmetric H3R2me2 mark is present. In active promoters, this modification is actively eliminated. H3R2me2a prevents WDR5 recognition, MLL methyltransferase recruitment, and H3K4 methylation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H3 Antibody (NB21-1002)(2)

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Product General Protocols

View specific protocols for Histone H3 Antibody (NB21-1002): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NB21-1002). (Showing 1 - 1 of 1 FAQs).

  1. I received H3R2me2a antibody from your company. I would like to use your antibody for western blotting. May I know the best blocking reagents (5% milk or 5%BSA) for this antibody?
    • When we generated our Western blot data with NB21-1002 we used a 4-15% gradient gel, and ran this in Tris-glycine buffer for one hour at 100V. The proteins were transferred to a 0.22uM nitrocellulose membrane, and this was blocked for one hour at room temperature with 3% BSA in TBS-T (1x TBS, 0.1% Tween-20). NB21-1002 was diluted to 1:1000 in blocking buffer and incubated at 4C overnight. The secondary antibody was incubated for one hour at room temperature.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB21-1002AF405
Alexa Fluor 488 Labeled NB21-1002AF488
Alexa Fluor 647 Labeled NB21-1002AF647
Alexa Fluor 700 Labeled NB21-1002AF700
Biotin Labeled NB21-1002B
DyLight 405 Labeled NB21-1002V
DyLight 488 Labeled NB21-1002G
DyLight 550 Labeled NB21-1002R
DyLight 650 Labeled NB21-1002C
FITC Labeled NB21-1002F
HRP Labeled NB21-1002H

Additional Histone H3 Products

Bioinformatics Tool for Histone H3 Antibody (NB21-1002)

Discover related pathways, diseases and genes to Histone H3 Antibody (NB21-1002). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on Histone H3.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Understanding the Reasons for Histone H3 K4 Trimethylation (H3K4Me3)
Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that...  Read full blog post.

The 'epi-genie' is Out of the Bottle: Functional Histone 3 Variants in Human Disease
Discovery of histone variants using highly specific antibodies has led to the emerging notion that alterations in histone modifications and further changes in chromatin structure are induced by exchange of histone variants. Covalent histone modificati...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Gene Symbol HIST2H3C