HIF-3 alpha Antibody


Western Blot: HIF-3 alpha Antibody [NB100-2287] - Analysis of HIF-3 alpha in Jurkat cell lysate.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB
1 mg/ml

HIF-3 alpha Antibody Summary

Bacterially expressed human HIF-3 alpha (N-terminus). [UniProt# Q9Y2N7]
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate, pH 7-8
0.1% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:100-1:200
Application Notes
This HIF-3 alpha antibody is useful for Western blot, where a band is observed ~70 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
70 kDa
Read Publications using
NB100-2287 in the following applications:

  • WB
    2 publications

Reactivity Notes

Mouse and human.

Alternate Names for HIF-3 alpha Antibody

  • Basic-helix-loop-helix-PAS protein MOP7
  • BHLHE17
  • bHLHe17HIF-3A2
  • Class E basic helix-loop-helix protein 17
  • HIF-3A4
  • HIF-3-alpha
  • HIF3-alpha
  • hypoxia inducible factor 3, alpha subunit
  • hypoxia-inducible factor 3-alpha
  • hypoxia-inducible factor-3 alpha 4
  • Inhibitory PAS domain protein
  • IPASHIF3-alpha-1
  • Member of PAS protein 7
  • MOP7PAS domain-containing protein 7


Hypoxia-inducible factor (HIF) is one of the most important factors in the cellular response to hypoxia, transcriptionally activating genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits, HIF1 alpha, HIF2 alpha, or HIF3 alpha. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation and cell survival. HIF3 alpha protein is one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha 3 subunit lacks the transactivation domain found in factors containing either the alpha 1 or alpha 2 subunits. HIF3 alpha may be a marker for tumor growth and angiogenesis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp, Ye
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, TCS, LA
Species: Hu, Mu, Rt, Bv, Ft, Pm, Sh
Applications: WB, ChIP, GS, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Fi, Ha, Pm, Sh
Applications: WB, Simple Western, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IP
Species: Hu, Mu, Rt, Dr, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
Species: Hu, Mu
Applications: WB
Species: Hu, Mu
Applications: WB, ICC/IF, IP
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P, IP
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB

Publications for HIF-3 alpha Antibody (NB100-2287)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 2 of 2.
Publications using NB100-2287 Applications Species
Jackson IL, Zhang X, Hadley C et al. Temporal expression of hypoxia-regulated genes is associated with early changes in redox status in irradiated lung Free Radic Biol Med 2012 Apr 25 [PMID:22588005] (WB, Mouse) WB Mouse
Tam, KKY. The role of hypoxia inducible factors in regulating ovarian function. Thesis (PhD) University of Adelaide, School of Paediatrics and Reproductive Health 2010 (WB, Mouse) WB Mouse

Reviews for HIF-3 alpha Antibody (NB100-2287) (0)

There are no reviews for HIF-3 alpha Antibody (NB100-2287). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HIF-3 alpha Antibody (NB100-2287). (Showing 1 - 1 of 1 FAQs).

  1. The customer wants to know the immunogen sequence.
    • NB100-2287 was made to a partial recombinant protein that is found between amino acids 1-275.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-2287AF405
Alexa Fluor (R) 488 Labeled NB100-2287AF488
Alexa Fluor (R) 647 Labeled NB100-2287AF647
Alexa Fluor (R) 700 Labeled NB100-2287AF700
Biotin Labeled NB100-2287B
DyLight 405 Labeled NB100-2287V
DyLight 488 Labeled NB100-2287G
DyLight 550 Labeled NB100-2287R
DyLight 650 Labeled NB100-2287C
FITC Labeled NB100-2287F
HRP Labeled NB100-2287H

Additional HIF-3 alpha Antibody Products

Related Products by Gene

Bioinformatics Tool for HIF-3 alpha Antibody (NB100-2287)

Discover related pathways, diseases and genes to HIF-3 alpha Antibody (NB100-2287). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HIF-3 alpha Antibody (NB100-2287)

Discover more about diseases related to HIF-3 alpha Antibody (NB100-2287).

Pathways for HIF-3 alpha Antibody (NB100-2287)

View related products by pathway.

PTMs for HIF-3 alpha Antibody (NB100-2287)

Learn more about PTMs related to HIF-3 alpha Antibody (NB100-2287).

Research Areas for HIF-3 alpha Antibody (NB100-2287)

Find related products by research area.

Blogs on HIF-3 alpha.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

HIF Prolyl Hydroxylase 2: an important Oxygen Sensor Protein
Prolyl hydroxylase domain (PHD) proteins, including PHD1, PHD2, and PHD3, mediate oxygen-dependent degradation of hypoxia-inducible factor (HIF) alpha subunits. Suppression of PHD enzymes leads to stabilization of HIFs and offers a potential treatment...  Read full blog post.

HIF Antibodies: Beyond HIF-1 alpha
The hypoxia inducible factors are a family of heterodimeric transcription factors which are activated in response to lowered oxygen levels, or hypoxia. Although it may seem that HIF-1 alpha receives all the attention, other HIF antibodies, such as the...  Read full blog post.

A Role for HIF-1 alpha Antibody in Renal Research
The Hypoxia Inducible Factors (HIFs) are a family of mammalian transcription factors which are expressed in response to low cellular oxygen concentrations (hypoxia). Three human hypoxia inducible factors have been identified, HIF-1, HIF-2 and HIF-3, e...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol HIF3A

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-2287 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought