BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free

Western Blot: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis using BCL2L2 mAb against HEK293 (1) and BCL2L2 (AA: 6-118)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis of Hela cells using BCL2L2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis of paraffin-embedded ovarian cancer tissues using BCL2L2 mouse mAb with DAB staining.

Flow Cytometry: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis of K562 cells using BCL2L2 mouse mAb (green) and negative control (red).

Western Blot: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis using BCL2L2 mAb against human BCL2L2 (AA: 6-118) recombinant protein. (Expected MW is 38.2 kDa)

Western Blot: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis using BCL2L2 mouse mAb against HCT116 (1), LOVO (2), SW480 (3), and HL-60 (4) cell lysate.

Immunohistochemistry: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis of paraffin-embedded cervical cancer tissues using BCL2L2 mouse mAb with DAB staining.

Flow Cytometry: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis of Hela cells using BCL2L2 mouse mAb (green) and negative control (red).

ELISA: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 4G12E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free Summary

• Description: Novus Biologicals Mouse BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free (NBP2-61706) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-BCL-W/BCL2L2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human BCL-W/BCL2L2 (AA: 6-118) expressed in E. Coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCL2L2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 20.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free

• Apoptosis regulator Bcl-W
• BCL2L2
• Bcl2-L-2
• BCL2-like 2
• Bclw
• Bcl-w
• BCLWbcl-2-like protein 2
• KIAA0271BCL2-L-2

Background

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCL-W/BCL2L2 Antibody (NBP2-61706)(1)

Publication using NBP2-61706

• Langendonk M, Smit N, Plattel W et al. Navitoclax Most Promising BH3 Mimetic for Combination Therapy in Hodgkin Lymphoma International Journal of Molecular Sciences 2022-11-09 [PMID: 36430230] (IHC-P, Human)

Bioinformatics

• Gene Symbol: BCL2L2