BCL-W/BCL2L2 Antibody (4G12E6)

Images

 
Western Blot: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis using BCL2L2 mAb against HEK293 (1) and BCL2L2 (AA: 6-118)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis of Hela cells using BCL2L2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with ...read more
Immunohistochemistry: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis of paraffin-embedded ovarian cancer tissues using BCL2L2 mouse mAb with DAB staining.
Flow Cytometry: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis of K562 cells using BCL2L2 mouse mAb (green) and negative control (red).
Western Blot: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis using BCL2L2 mAb against human BCL2L2 (AA: 6-118) recombinant protein. (Expected MW is 38.2 kDa)
Western Blot: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis using BCL2L2 mouse mAb against HCT116 (1), LOVO (2), SW480 (3), and HL-60 (4) cell lysate.
Immunohistochemistry: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis of paraffin-embedded cervical cancer tissues using BCL2L2 mouse mAb with DAB staining.
Flow Cytometry: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis of Hela cells using BCL2L2 mouse mAb (green) and negative control (red).
ELISA: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
4G12E6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

BCL-W/BCL2L2 Antibody (4G12E6) Summary

Immunogen
Purified recombinant fragment of human BCL2L2 (AA: 6-118) expressed in E. Coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
BCL2L2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
20.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
BCL2L2-PABPN1 Knockout 293T Cell Lysate
BCL-W/BCL2L2 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for BCL-W/BCL2L2 Antibody (4G12E6)

  • Apoptosis regulator Bcl-W
  • BCL2L2
  • Bcl2-L-2
  • BCL2-like 2
  • Bclw
  • Bcl-w
  • BCLWbcl-2-like protein 2
  • KIAA0271BCL2-L-2

Background

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BCL2L2