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HGF Antibody [Unconjugated]

Images

 
Recombinant Human HGF (Catalog # 294-HGN) induces IL-11 secretion in the Saos-2 human osteosarcoma cell line. The dose-dependent response (orange line) is measured by the Human IL-11 Duoset (Catalog # DY218). Under ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

HGF Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGF (R&D Systems, Catalog # 294-HG)
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
Accession # P14210
Specificity
Detects human HGF in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse HGF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HGF
Purity Statement
Protein A or G purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Neutralization <2 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AB-294-NA in the following applications:

Publications
Read Publications using
AB-294-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HGF Antibody [Unconjugated]

  • deafness, autosomal recessive 39
  • DFNB39
  • EC 3.4.21
  • EC 3.4.21.7
  • fibroblast-derived tumor cytotoxic factor
  • F-TCF
  • hepatocyte growth factor (hepapoietin A; scatter factor)
  • Hepatopoeitin-A
  • Hepatopoietin A
  • HGF
  • HGFB
  • HPTA
  • HPTAhepatocyte growth factor
  • lung fibroblast-derived mitogen
  • Scatter factor
  • SF
  • SFhepatopoeitin-A

Background

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity. Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF-dependent c-MET activation is implicated in the development of many human cancers. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription. This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis. HGF can also alter epithelium morphology by the induction of nectin-1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions. In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake. HGF promotes the motility of cardiac stem cells in damaged myocardium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HGF Antibody (AB-294-NA)(10)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 4 applications: ELISA Development, IHC, Neutralization, Western Blot.


Filter By Application
ELISA Development
(1)
IHC
(1)
Neutralization
(3)
Western Blot
(1)
All Applications
Filter By Species
Human
(3)
Rat
(2)
All Species
Showing Publications 1 - 10 of 10.
Publications using AB-294-NA Applications Species
A Elgaabari, N Imatomi, H Kido, M Seki, S Tanaka, Y Matsuyoshi, T Nakashima, S Sawano, W Mizunoya, T Suzuki, M Nakamura, JE Anderson, R Tatsumi A pilot study on nitration/dysfunction of NK1 segment of myogenic stem cell activator HGF Biochemistry and Biophysics Reports, 2022-06-11;31(0):101295. 2022-06-11 [PMID: 35721345] (IHC, Western Blot, Rat) IHC, Western Blot Rat
Yoko Yamaguchi, Akira Saito, Masafumi Horie, Akira Aoki, Patrick Micke, Mitsuhiro Ohshima et al. Targeting hepatocyte growth factor in epithelial–stromal interactions in an in vitro experimental model of human periodontitis Odontology 2021-10-01 [PMID: 34128105]
SJ Wang, R Li, TSC Ng, G Luthria, MJ Oudin, M Prytyskach, RH Kohler, R Weissleder, DA Lauffenbur, MA Miller Efficient blockade of locally reciprocated tumor-macrophage signaling using a TAM-avid nanotherapy Sci Adv, 2020-05-22;6(21):eaaz8521. 2020-05-22 [PMID: 32494745] (Neutralization, Human) Neutralization Human
Sohinee Bhattacharyya, Chet Oon, Aayush Kothari, Wesley Horton, Jason Link, Rosalie C. Sears et al. Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer Journal of Experimental Medicine 2020-08-03 [PMID: 32434218]
N Kanaji, M Yokohira, Y Nakano-Nar, N Watanabe, K Imaida, N Kadowaki, S Bandoh Hepatocyte growth factor produced in lung fibroblasts enhances non-small cell lung cancer cell survival and tumor progression Respir. Res., 2017-06-15;18(1):118. 2017-06-15 [PMID: 28619066] (Neutralization, Human) Neutralization Human
Kouichi Sakamoto, Ngin Cin Khai, Yuqing Wang, Rie Irie, Hideo Takamatsu, Hiroshi Matsufuji et al. Heparin-binding epidermal growth factor-like growth factor and hepatocyte growth factor inhibit cholestatic liver injury in mice through different mechanisms International Journal of Molecular Medicine 2016-12-01 [PMID: 27779646]
M Huang, T Liu, P Ma, RA Mitteer, Z Zhang, HJ Kim, E Yeo, D Zhang, P Cai, C Li, L Zhang, B Zhao, L Roccogrand, DM O'Rourke, N Dahmane, Y Gong, C Koumenis, Y Fan c-Met-mediated endothelial plasticity drives aberrant vascularization and chemoresistance in glioblastoma J Clin Invest, 2016-04-04;0(0):. 2016-04-04 [PMID: 27043280] (Neutralization, Human) Neutralization Human
Jing Bai, Ting-Yuan Tu, Choong Kim, Jean Paul Thiery, Roger D. Kamm Identification of drugs as single agents or in combination to prevent carcinoma dissemination in a microfluidic 3D environment Oncotarget 2015-11-03 [PMID: 26474384]
Jianying Zhang, Kellie K. Middleton, Freddie H. Fu, Hee-Jeong Im, James H-C. Wang HGF Mediates the Anti-inflammatory Effects of PRP on Injured Tendons PLoS ONE 2013-06-28 [PMID: 23840657]
Tatsumi R, Wuollet AL, Tabata K, Nishimura S, Tabata S, Mizunoya W, Ikeuchi Y, Allen RE A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation. Am. J. Physiol., Cell Physiol., 2009-01-21;296(4):C922-9. 2009-01-21 [PMID: 19158401] (ELISA Development, Rat) ELISA Development Rat

Review for HGF Antibody (AB-294-NA) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using AB-294-NA:
Filter by Applications
WB
(1)
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Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Rat 01/05/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 23035711
SpeciesRat

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for HGF Antibody (AB-294-NA) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on HGF.

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The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.

VEGFR-2 - A highly active kinase
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Verified Customer
01/05/2015
Application: WB
Species: Rat

Bioinformatics

Gene Symbol HGF
Uniprot