Heparanase/HPSE Antibody (4D7)

ELISA: Heparanase/HPSE Antibody (4D7) [H00010855-M05] - Detection limit for recombinant GST tagged HPSE is approximately 0.3ng/ml as a capture antibody.

Product Discontinued

Heparanase/HPSE Antibody (4D7) Summary

• Immunogen: HPSE (NP_006656.1, 72 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LILLGSPKLRTLARGLSPAYLRFGGTKTDFLIFDPKKESTFEERSYWQSQVNQDICKYGSIPPDVEEKLRLEWPYQEQLLLREHYQKKFKNSTYSRSSV
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HPSE
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Western Blot 1:100 - 1:2000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Heparanase/HPSE Antibody (4D7)

• EC 3.2
• Endo-glucoronidase
• HEP
• Heparanase
• Heparanase-1
• HPA1
• HPAheparanase exon 9 and 10 deletion
• HPR1
• HPSE
• HPSE1heparanase-1
• HSE1
• HSE1heparanase exon10-deletion

Background

Heparanase is an endo-beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasations of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and an 8 kDa subunit. It is produced as a latent 65 kDa precursor and proteolytically processed to its active form. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HPSE