Hemoglobin A1 Antibody (C7) - BSA Free

Western Blot: Hemoglobin A1 Antibody (C7) [NBP3-42212] - Sample: Human Serum Primary Ab: 2ug/ml NBP3-42212; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Immunohistochemistry-Paraffin: Hemoglobin A1 Antibody (C7) [NBP3-42212] - DAB staining on IHC-P; Sample: Human Placenta Tissue; Primary Ab: 30ug/ml NBP3-42212; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Western Blot: Hemoglobin A1 Antibody (C7) [NBP3-42212] - Sample: Human Serum Primary Ab: 2ug/ml NBP3-42212; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

• Reactivity: Hu
• Applications: WB, IHC
• Clone: C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Hemoglobin A1 Antibody (C7) - BSA Free Summary

• Immunogen: Immunized with recombinant Human Hemoglobin A1 (Uniprot#: P69905; Met1-Arg142 (Uniprot#: P69905)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HBA1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-20ug/mL
  • Immunohistochemistry-Paraffin 5-20ug/mL
  • Western Blot 0.5-2ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.01M PBS, pH7.4, 50% glycerol
• Preservative: 0.05% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Hemoglobin A1 Antibody (C7) - BSA Free

• alpha one globin
• alpha-1 globin
• alpha-1-globin
• alpha-globin
• CD31
• hemoglobin alpha 1 globin chain
• Hemoglobin alpha chain
• hemoglobin alpha-1 chain
• hemoglobin subunit alpha
• hemoglobin, alpha 1
• MGC126895
• MGC126897

Background

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HBA1