![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NBP1-78981] - HDAC-6 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78981-img0001.jpg "Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NBP1-78981] - HDAC-6 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC (-), WB (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.07 mg/ml |
| Immunogen | A synthetic peptide made to a C-terminal portion of the human HDAC6 protein (between residues 1175-1215). [UniProt Q9UBN7] |
| Localization | Nucleus. Cytoplasm. Note: It is cytoplasmic when associated with microtubules. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HDAC6 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody did not work in IHC-paraffin embedded sections in mouse intestine or Western blot. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 30% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.07 mg/ml |
| Purity | Immunogen affinity purified |
![Immunohistochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunohistochemistry_20892.jpg)
![Immunocytochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunocytochemistry__Immunofluorescence_21143.jpg)
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| Publication using NBP1-78981 | Applications | Species |
|---|---|---|
| Kumar A, Larrea D, Pero ME et al. MFN2-dependent recruitment of ATAT1 coordinates mitochondria motility with alpha-tubulin acetylation and is disrupted in CMT2A bioRxiv : the preprint server for biology 2023-03-16 [PMID: 36993604] (WB, ICC/IF, Mouse) | WB, ICC/IF | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for HDAC6 Antibody (NBP1-78981)Find related products by research area.
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LAMP2: Protector of the lysosome LAMP2 belongs to the family of membrane glycoproteins who confer selectins with carbohydrate ligands. LAMP2 has been implicated in tumor cell metastasis, as well as overall protection, maintenance, and adhesion of the lysosome. It appears that LAMP2 m... Read full blog post. |
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![Flow Cytometry: HDAC6 Antibody [NBP1-78981] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP1-78981. Staining of isolated T-cells. Image from verified customer review (Andressa Sodre, Moffitt Cancer Center).](http://images.novusbio.com/fullsize/HDAC6-Antibody-Flow-Cytometry-NBP1-78981-img0003.jpg "Flow Cytometry: HDAC6 Antibody [NBP1-78981] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP1-78981. Staining of isolated T-cells. Image from verified customer review (Andressa Sodre, Moffitt Cancer Center).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")