HDAC5 Antibody

Western Blot: HDAC5 Antibody [NBP2-03988] - Analysis of HDAC-5 in NIH 3T3 cell lysate using this antibody at 2 ug/ml.

Product Discontinued

HDAC5 Antibody Summary

• Immunogen: This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 572-596 of human HDAC-5.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1-3 ug/ml
• Application Notes: Chromatin Immunoprecipitation: Refer to Imbriano (2005, Fig 7) for details.

Reactivity Notes

Human and Mouse

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for HDAC5 Antibody

• Antigen NY-CO-9
• EC 3.5.1.98
• FLJ90614
• HD5
• Histone Deacetylase 5
• KIAA0600
• NY-CO-9

Background

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of HDAC family have been identified in the past several years. These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns. These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HDAC5 Antibody (NBP2-03988)(1)

Publication using NBP2-03988

• Imbriano C, Gurtner A, Cocchiarella F et al. Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters. Mol Cell Biol. 2005-05-01 [PMID: 15831478]

Bioinformatics

• Gene Symbol: HDAC5
• Uniprot:
  • NP_001015053(Human)