HDAC10 Antibody - N-terminal

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

HDAC10 Antibody - N-terminal Summary

• Immunogen: A synthetic peptide near the N-terminus of human HDAC10 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Application Notes: This antibody is tested by Immunohistochemistry and useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~71kDa corresponding to the appropriate cell lysate or extract.

Reactivity Notes

BLAST analysis was used to suggest cross-reactivity with human based on 100% sequence homology. Cross-reactivity with HDAC10 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for HDAC10 Antibody - N-terminal

• DKFZP761B039
• EC 3.5.1.48
• EC 3.5.1.62
• EC 3.5.1.98
• HD10
• HDAC10
• histone deacetylase 10
• MGC149722

Background

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC10