![Western Blot: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Analysis of HBG1/2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: human placenta tissue lysateLane 2: human brain tissue lysate](http://images.novusbio.com/fullsize/Hemoglobin-gamma-A-Antibody-JM84-10-Western-Blot-NBP2-66885-img0007.jpg "Western Blot: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Analysis of HBG1/2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: human placenta tissue lysateLane 2: human brain tissue lysate")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | JM84-10 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit HBG1/2 Antibody (JM84-10) (NBP2-66885) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human HBG1/2 aa 2-45 / 147. (SwissProt: P69891 Human; SwissProt: P69892 Human) |
| Localization | Cytosol, hemoglobin complex. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | HBG1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for HBG1/2 Antibody (NBP2-66885)Find related products by research area.
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| Gene Symbol | HBG1 |
![Immunocytochemistry/Immunofluorescence: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Staining HBG1/2 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Hemoglobin-gamma-A-Antibody-JM84-10-Immunocytochemistry-Immunofluorescence-NBP2-66885-img0006.jpg "Immunocytochemistry/Immunofluorescence: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Staining HBG1/2 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Analysis of paraffin-embedded human tonsil tissue using anti-HBG1/2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Hemoglobin-gamma-A-Antibody-JM84-10-Immunohistochemistry-Paraffin-NBP2-66885-img0003.jpg "Immunohistochemistry-Paraffin: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Analysis of paraffin-embedded human tonsil tissue using anti-HBG1/2 antibody. Counter stained with hematoxylin.")
![Immunocytochemistry/Immunofluorescence: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Hemoglobin gamma A Antibody (JM84-10) [NBP2-66885] - Staining HBG1/2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Hemoglobin-gamma-A-Antibody-JM84-10-Immunocytochemistry-Immunofluorescence-NBP2-66885-img0001.jpg "Immunocytochemistry/Immunofluorescence: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Hemoglobin gamma A Antibody (JM84-10) [NBP2-66885] - Staining HBG1/2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Staining HBG1/2 in D3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Hemoglobin-gamma-A-Antibody-JM84-10-Immunocytochemistry-Immunofluorescence-NBP2-66885-img0005.jpg "Immunocytochemistry/Immunofluorescence: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Staining HBG1/2 in D3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Analysis of paraffin-embedded human spleen tissue using anti-HBG1/2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Hemoglobin-gamma-A-Antibody-JM84-10-Immunohistochemistry-Paraffin-NBP2-66885-img0002.jpg "Immunohistochemistry-Paraffin: HBG1/2 Antibody (JM84-10) [NBP2-66885] - Analysis of paraffin-embedded human spleen tissue using anti-HBG1/2 antibody. Counter stained with hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")