Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free [NBP3-25499] - Hemoglobin epsilon antibody [HL2457] detects Hemoglobin epsilon protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hemoglobin epsilon stained by Hemoglobin epsilon antibody [HL2457] (NBP3-25499) diluted at 1:500. Blue: Fluoroshield with DAPI .

Immunohistochemistry-Paraffin: Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free [NBP3-25499] - Hemoglobin epsilon antibody [HL2457] detects secreted Hemoglobin epsilon protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. Hemoglobin epsilon stained by Hemoglobin epsilon antibody [HL2457] (NBP3-25499) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free [NBP3-25499] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [HL2457] (NBP3-25499) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemistry-Paraffin: Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free [NBP3-25499] - Hemoglobin epsilon antibody [HL2457] detects secreted Hemoglobin epsilon protein by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. Hemoglobin epsilon stained by Hemoglobin epsilon antibody [HL2457] (NBP3-25499) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: HL2457
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free (NBP3-25499) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide of human Hemoglobin epsilon.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HBE1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free

• epsilon globin
• Epsilon-globin
• HBEepsilon-globin
• Hemoglobin epsilon chain
• hemoglobin subunit epsilon
• hemoglobin, epsilon 1

Background

The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with twozeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together withtwo alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal,and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in thefollowing order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3' (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HBE1