HAND1 Antibody [Unconjugated]

HAF017). A specific band was detected for HAND1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF017). A specific band was detected for HAND1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HAND1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." > NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." title="HAND1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." />

HAND1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

<b>Left:</b> Simple Western lane view shows lysates of human cerebellum, loaded at 1 mg/ml. A specific band was detected for HAND1 at approximately 26 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # 043-522-2). This experiment was conducted under reducing conditions and using the 2-40kDa separation system. <b>Right:</b> Simple Western electropherogram showing the same Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the human cerebellum.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

HAND1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human HAND1
  Met1-Gln215
  Accession # O96004
• Specificity: Detects human HAND1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human HAND2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HAND1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 10-50 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HAND1 Antibody [Unconjugated]

• BHLHA27
• bHLHa27heart- and neural crest derivatives-expressed protein 1
• Class A basic helix-loop-helix protein 27
• eHand
• Extraembryonic tissues, heart, autonomic nervous system and neural crestderivatives-expressed protein 1
• HAND1
• heart and neural crest derivatives expressed 1
• Hxt
• Thing1

Background

HAND1 belongs to the HAND family of conserved basic helix-loop-helix transcription factors. It is expressed in the heart, ganglia, neural crest derivatives and extraembryonic tissues. HAND1 plays an essential role in heart development and is critical for the specification of extraembryonic tissues including trophoblasts. Human and mouse HAND1 share approximately 92% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HAND1 Antibody (AF3168)(14)

Publications using AF3168

• Okubo, C;Nakamura, M;Sato, M;Shichino, Y;Mito, M;Takashima, Y;Iwasaki, S;Takahashi, K; EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency Science advances 2025-04-11 [PMID: 40203091] (Immunocytochemistry, Human)
• Stanberry, I;Cunningham, D;Ye, S;Alonzo, M;Zhao, MT;Garg, V;Lilly, B; Characterization of an induced pluripotent stem cell line NCHi011-A from a 23-year-old female with Alagille Syndrome harboring a heterozygous JAG1 pathogenic variant Stem cell research 2023-09-23 [PMID: 37774637] (ICC, Human)
• Cunningham, D;Stanberry, I;Ye, S;Alonzo, M;Zhao, MT;Garg, V;Lilly, B; Generation of iPSC line NCHi012-A from a patient with Alagille syndrome and heterozygous pathogenic variant in the JAG1 gene Stem cell research 2023-08-01 [PMID: 37549562] (ICC, Human)
• Marina Gattiglio, Michelle Protzek, Christian Schröter Population-level antagonism between FGF and BMP signaling steers mesoderm differentiation in embryonic stem cells Biology Open 2023-08-15 [PMID: 37530863]
• Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelse, Sanne Hillenius, Talia Van Der Helm et al. Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay Cell Reports Methods 2023-06-26 [PMID: 37426764] (Immunocytochemistry, Human)
• Aracely Castillo-Venzor, Christopher A Penfold, Michael D Morgan, Walfred WC Tang, Toshihiro Kobayashi, Frederick CK Wong et al. Origin and segregation of the human germline Life Science Alliance 2023-08-01 [PMID: 37217306] (Immunohistochemistry, Human)
• S Teague, G Primavera, B Chen, E Freeburne, H Khan, K Jo, C Johnson, I Heemskerk The time integral of BMP signaling determines fate in a stem cell model for early human development bioRxiv : the preprint server for biology, 2023-04-10;0(0):. 2023-04-10 [PMID: 37090515] (ICC, Human)
• EY Akda?, S Turan, D Guhathakur, A Ekici, S Salar, DC Lie, B Winner, A Fejtova CRISPR/Cas9-mediated generation of hESC lines with homozygote and heterozygote p.R331W mutation in CTBP1 to model HADDTS syndrome Stem Cell Research, 2022-12-30;67(0):103012. 2022-12-30 [PMID: 36610307] (Flow Cytometry, ICC, Human)
• H Lin, SQ Ye, ZH Xu, JS Penaloza, M Aljuhani, T Vetter, MT Zhao, KL McBride Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD) Stem Cell Research, 2022-10-31;65(0):102958. 2022-10-31 [PMID: 36343514] (ICC, Human)
• M Ohgushi, N Taniyama, A Vandenbon, M Eiraku Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model Cell Reports, 2022-06-21;39(12):110973. 2022-06-21 [PMID: 35732132] (Flow Cytometry, ICC, Human)
• Kazutoshi Takahashi, Chikako Okubo, Michiko Nakamura, Mio Iwasaki, Yuka Kawahara, Tsuyoshi Tabata et al. A stress-reduced passaging technique improves the viability of human pluripotent cells Cell Reports Methods 2022-02-28 [PMID: 35474962]
• A Mehta, P Lu, BV Taylor, J Charleswor, AL Cook, KP Burdon, AW Hewitt, KM Young Generation of MNZTASi001-A, a human pluripotent stem cell line from a person with primary progressive multiple sclerosis Stem Cell Research, 2021-10-12;57(0):102568. 2021-10-12 [PMID: 34678664] (Immunocytochemistry, Human)
• M Zheng, S Erhardt, D Ai, J Wang Bmp Signaling Regulates Hand1 in a Dose-Dependent Manner during Heart Development International Journal of Molecular Sciences, 2021-09-11;22(18):. 2021-09-11 [PMID: 34576009] (Western Blot, Mouse)
• S Chhabra, L Liu, R Goh, X Kong, A Warmflash Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids PLoS Biol., 2019-10-15;17(10):e3000498. 2019-10-15 [PMID: 31613879] (ICC, Human)

Bioinformatics

• Gene Symbol: HAND1
• Uniprot:
  • O96004()