GRP78/HSPA5 Antibody


Western Blot: GRP78/HSPA5 Antibody [NB300-520] - Figure 1 shows a Western blot of GRP78 on indicated cell extracts using NB300-520.
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NB300-520] - GRP78/BIP antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight more
Simple Western: GRP78/HSPA5 Antibody [NB300-520] - Simple Western lane view shows a specific band for GRP78 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa more

Product Details

Reactivity Hu, Mu, Rt, Pm(-)Species Glossary
Applications WB, Simple Western, ICC/IF, IHC-P
1 mg/ml

GRP78/HSPA5 Antibody Summary

A synthetic peptide made to a C-terminal portion of rat GRP78 (within residues 600-654). [UniProt# P06761]
ER Stress Marker
Immunogen affinity purified
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Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.05% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 2 ug/ml
  • Simple Western 1:25
  • Immunocytochemistry/Immunofluorescence 1:20
  • Immunohistochemistry-Paraffin
Application Notes
This GRP78 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot this antibody detects a 78 kDa protein representing GRP 78 from rat liver extract and HeLa cells. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 27106802).
Theoretical MW
78 kDa
Read Publications using
NB300-520 in the following applications:

  • 1 publication
  • WB
    3 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26865610). Mouse and rat. Does not cross-react with African Green Monkey (COS cells).

Alternate Names for GRP78/HSPA5 Antibody

  • 78 kDa glucose-regulated protein
  • BIP
  • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
  • FLJ26106
  • GRP-78
  • GRP78BIP
  • Heat shock 70 kDa protein 5
  • heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
  • heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
  • HSP70-5
  • HSPA5
  • Immunoglobulin heavy chain-binding protein
  • MIF2


Within the ER misfolded proteins are detected by Bip(GPR78). In the presence of misfolded proteins, Bip disscociates from PERK, allowing it to homodimerize and autophosphoyate. In its dimerized, phosphorylated form PERK phosphorylates eIF2. The phosphorylation of eIF2 blocks the majority of translation to prevent the continued accumulation of protein in the ER. Bip also is binds to IRE1 and ATF6 under normal ER conditions, but dissociates upon accumulation of misfolded proteins. Unbound ATF6 is translocated to the gogli where it is converted into a cleaved, active form. The cleaved form of ATF6 is then able to up regulated transcription of UPR genes including XBP1. Activated IRE1 acts as an endoribonuclease to an intron from XBP1 transcripts. The XBP1 splice variant codes for an active transcription factor which activates transcription of P58IPK. P58IPK is a HSP40 protein which binds to and inhibits PERK. Thus, if the accumulated, misfolded proteins have been removed, P58IPK acts to shut down the UPR by removing the block to translation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GRP78/HSPA5 Antibody (NB300-520)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB300-520AF405
Alexa Fluor (R) 488 Labeled NB300-520AF488
Alexa Fluor (R) 647 Labeled NB300-520AF647
Alexa Fluor (R) 700 Labeled NB300-520AF700
Biotin Labeled NB300-520B
DyLight 405 Labeled NB300-520V
DyLight 488 Labeled NB300-520G
DyLight 550 Labeled NB300-520R
DyLight 650 Labeled NB300-520C
FITC Labeled NB300-520F
HRP Labeled NB300-520H

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Blogs on GRP78/HSPA5.

GRP78 - molecular chaperone and negative regulator of the unfolded protein response
The 78 kDa glucose-regulated protein (GRP78) is the eukaryotic orthologue to the prokaryotic heat shock 70 kDa protein 5 (HSPA5). GRP78 is also sometimes referred to as BiP. GRP78 is a member of the HSP70 family and plays dynamic roles in protein...  Read full blog post.

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Gene Symbol HSPA5

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