Western Blot: GRP78/HSPA5 Antibody [NB300-520] - Figure 1 shows a Western blot of GRP78 on indicated cell extracts using NB300-520.
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody [NB300-520] - GRP78/BIP antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Simple Western: GRP78/HSPA5 Antibody [NB300-520] - Simple Western lane view shows a specific band for GRP78 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa ...read more
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.05% Sodium Azide
Immunogen affinity purified
Western Blot 2 ug/ml
Simple Western 1:25
This GRP78 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot this antibody detects a 78 kDa protein representing GRP 78 from rat liver extract and HeLa cells. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 27106802).
Human reactivity reported in scientific literature (PMID: 26865610). Mouse and rat. Does not cross-react with African Green Monkey (COS cells).
Alternate Names for GRP78/HSPA5 Antibody
78 kDa glucose-regulated protein
Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
Heat shock 70 kDa protein 5
heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Immunoglobulin heavy chain-binding protein
Within the ER misfolded proteins are detected by Bip(GPR78). In the presence of misfolded proteins, Bip disscociates from PERK, allowing it to homodimerize and autophosphoyate. In its dimerized, phosphorylated form PERK phosphorylates eIF2. The phosphorylation of eIF2 blocks the majority of translation to prevent the continued accumulation of protein in the ER. Bip also is binds to IRE1 and ATF6 under normal ER conditions, but dissociates upon accumulation of misfolded proteins. Unbound ATF6 is translocated to the gogli where it is converted into a cleaved, active form. The cleaved form of ATF6 is then able to up regulated transcription of UPR genes including XBP1. Activated IRE1 acts as an endoribonuclease to an intron from XBP1 transcripts. The XBP1 splice variant codes for an active transcription factor which activates transcription of P58IPK. P58IPK is a HSP40 protein which binds to and inhibits PERK. Thus, if the accumulated, misfolded proteins have been removed, P58IPK acts to shut down the UPR by removing the block to translation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.