GRP78/HSPA5 Antibody - BSA Free

Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in A) HeLa, B) human liver, C) mouse liver, and D) rat liver lysate using NB100-56411 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were use for this test.

Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in formalin-fixed, paraffin-embedded mouse liver tissue using this antibody at 5 ug/ml.

Western Blot: GRP78/HSPA5 Antibody [NB100-56411] - Analysis of GRP78/HSPA5 in mouse liver tissue enriched using Endoplasmic Reticulum Enrichment Kit and 1 ug/ml of NB100-56411 and 0.25 ug/ml of anti-GAPDH loading control. Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction.

Simple Western: GRP78/HSPA5 Antibody [NB100-56411] - Simple Western lane view shows a specific band for GRP78/HSPA5 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GRP78/HSPA5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GRP78/HSPA5 Antibody - BSA Free (NB100-56411) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-GRP78/HSPA5 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 550-600 of human GRP78 was used as the immunogen.
• Marker: ER Stress Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSPA5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500. Use reported in scientific literature (Egawa et al (2011))
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Simple Western 1:250
  • Western Blot 1-3 ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:250, apparent MW was 77 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reactivity Notes

Predicted to react with Chimpanzee, Canine, Macaque, Rat, Xenopus and Zebrafish.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for GRP78/HSPA5 Antibody - BSA Free

• 78 kDa glucose-regulated protein
• BIP
• Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
• FLJ26106
• GRP78
• GRP-78
• GRP78BIP
• Heat shock 70 kDa protein 5
• heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
• heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• HSP70-5
• HSPA5
• Immunoglobulin heavy chain-binding protein
• MIF2

Background

GRP78, a 78 kDa glucose-regulated protein, also known as BiP or immunoglobulin heavy chain binding protein, is a stress-response protein which is induced by agents or conditions that adversely affect endoplasmic reticulum (ER) function. This protein is essential for the proper glycosylation, folding and assembly of many membrane bound and secreted proteins. GRP78 is critical for maintenance of cell homeostasis and the prevention of apoptosis. GRP78 protein levels have been shown to be a reliable biomarker of hypoglycermia as well as serving a neuroprotective function in neurons exposed to glutamate and oxidative stress. GRP78 levels are reduced in the brains of Alzheimer's Disease patients and decreased expression of GRP78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. The induction of the GRP78 protein has been associated with the development of drug-resistance to antitumor drugs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GRP78/HSPA5 Antibody (NB100-56411)(4)

Publications using NB100-56411

• Li T, Yang Y, Li Y et al. Platelets mediate inflammatory monocyte activation by SARS-CoV-2 Spike protein The Journal of clinical investigation 2021-12-29 [PMID: 34964720] (WB, Human)
• Egawa N, Yamamoto K, Inoue H et al. The endoplasmic reticulum stress sensor, ATF6a, protects against neurotoxin-induced dopaminergic neuronal death. J Biol Chem. 2011-03-11 [PMID: 21131360] (WB, ICC/IF, Mouse)
  
  Details:
  BiP/GRP78 (IMG-5228A). WB & IF/ICC: Mouse midbrain or striatum from ATF6 alpha WT +/+ or ATF6 alpha kO -/- mice, Fig 2B.
• Panaretakis Theocharis, Kepp Oliver, Brockmeier Ulf et al. Mechanisms of pre-apoptotic calreticulin exposure in immunogenic cell death. EMBO J. 2009-03-04 [PMID: 19165151] (Human)
• Sharma A, Masri J, Jo OD et al. Protein kinase C regulates internal initiation of translation of the GATA-4 mRNA following vasopressin-induced hypertrophy of cardiac myocytes. J Biol Chem. 2007-03-30 [PMID: 17284439] (WB)
  
  Details:
  BiP/GRP78 (IMG-5228A). WB: H9c2 ventricular cardiomyocytes treated with arginine vasopressin (AVP), Fig 8A.

Bioinformatics

• Gene Symbol: HSPA5
• Uniprot:
  • P11021(Human)