GRIM19 Antibody

Images

 
Western Blot: GRIM19 Antibody [NB100-56621] - Analysis of GRIM-19 in A) human heart and B) HeLa cell lysate using GRIM-19 antibody at 1:500. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution ...read more
Immunohistochemistry-Paraffin: GRIM19 Antibody [NB100-56621] - Analysis of human liver using GRIM-19 antibody at 1:100.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GRIM19 Antibody Summary

Immunogen
This antibody was developed by immunizing rabbits with a mixture of synthetic peptides of human GRIM-19.
Specificity
In HeLa, an approx. 16 kDa band is observed
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NDUFA13
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100
Positive Control
HeLa Lysate (NB800-PC1)
GRIM19 Lysate (NBL1-13538)

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera with PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for GRIM19 Antibody

  • B16.6
  • CDA016
  • Cell death regulatory protein GRIM-19
  • cell death-regulatory protein GRIM19
  • CI-B16.6
  • complex I B16.6 subunit
  • Complex I-B16.6
  • FLJ58045
  • FLJ59191
  • Gene associated with retinoic and IFN-induced mortality 19 protein
  • Gene associated with retinoic and interferon-induced mortality 19 protein
  • GRIM-19CGI-39
  • GRIM19NADH-ubiquinone oxidoreductase B16.6 subunit
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13
  • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

Background

Mitochondrial NADH:ubiquinone oxidoreductase (complex I) catalyzes the first step in the electron transport chain, the oxidation of NADH to NAD+ coupled to proton translocation across the inner mitochondrial membrane. GRIM-19 is a component of the complex I and is a product of a cell death regulatiry gene induced by interferon-b and retinoic acid. GRIM-19 has previously been detected in HeLa cells, predominantly in the nucleus, though punctate staining of the cytoplasm was also observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NDUFA13
Entrez
Uniprot