GRIM19 Antibody

Western Blot: GRIM19 Antibody [NB100-56621] - Analysis of GRIM-19 in A) human heart and B) HeLa cell lysate using GRIM-19 antibody at 1:500. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: GRIM19 Antibody [NB100-56621] - Analysis of human liver using GRIM-19 antibody at 1:100.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GRIM19 Antibody Summary

• Immunogen: This antibody was developed by immunizing rabbits with a mixture of synthetic peptides of human GRIM-19.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFA13
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:100 - 1:1000
• Application Notes: In HeLa, an approx. 16 kDa band is observed
• Control:

  HeLa Whole Cell Lysate
  GRIM19 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera with PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for GRIM19 Antibody

• B16.6
• CDA016
• Cell death regulatory protein GRIM-19
• cell death-regulatory protein GRIM19
• CI-B16.6
• complex I B16.6 subunit
• Complex I-B16.6
• FLJ58045
• FLJ59191
• Gene associated with retinoic and IFN-induced mortality 19 protein
• Gene associated with retinoic and interferon-induced mortality 19 protein
• GRIM-19CGI-39
• GRIM19NADH-ubiquinone oxidoreductase B16.6 subunit
• NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13
• NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

Background

Mitochondrial NADH:ubiquinone oxidoreductase (complex I) catalyzes the first step in the electron transport chain, the oxidation of NADH to NAD+ coupled to proton translocation across the inner mitochondrial membrane. GRIM-19 is a component of the complex I and is a product of a cell death regulatiry gene induced by interferon-b and retinoic acid. GRIM-19 has previously been detected in HeLa cells, predominantly in the nucleus, though punctate staining of the cytoplasm was also observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFA13
• Entrez:
  • 51079(Human)
• Uniprot:
  • Q9P0J0(Human)