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GRIM19 Antibody

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Western Blot: GRIM19 Antibody [NB100-56621] - Analysis of GRIM-19 in A) human heart and B) HeLa cell lysate using GRIM-19 antibody at 1:500. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Western Blot: GRIM19 Antibody [NB100-56621] - Analysis of GRIM-19 in A) human heart and B) HeLa cell lysate using GRIM-19 antibody at 1:500. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution ...read more
Immunohistochemistry-Paraffin: GRIM19 Antibody [NB100-56621] - Analysis of human liver using GRIM-19 antibody at 1:100.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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GRIM19 Antibody Summary

Immunogen
This antibody was developed by immunizing rabbits with a mixture of synthetic peptides of human GRIM-19.
Specificity
In HeLa, an approx. 16 kDa band is observed
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NDUFA13
Purity
Unpurified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
Control
HeLa Whole Cell Lysate
GRIM19 Overexpression Lysate

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera with PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for GRIM19 Antibody

  • B16.6
  • CDA016
  • Cell death regulatory protein GRIM-19
  • cell death-regulatory protein GRIM19
  • CI-B16.6
  • complex I B16.6 subunit
  • Complex I-B16.6
  • FLJ58045
  • FLJ59191
  • Gene associated with retinoic and IFN-induced mortality 19 protein
  • Gene associated with retinoic and interferon-induced mortality 19 protein
  • GRIM-19CGI-39
  • GRIM19NADH-ubiquinone oxidoreductase B16.6 subunit
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13
  • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

Background

Mitochondrial NADH:ubiquinone oxidoreductase (complex I) catalyzes the first step in the electron transport chain, the oxidation of NADH to NAD+ coupled to proton translocation across the inner mitochondrial membrane. GRIM-19 is a component of the complex I and is a product of a cell death regulatiry gene induced by interferon-b and retinoic acid. GRIM-19 has previously been detected in HeLa cells, predominantly in the nucleus, though punctate staining of the cytoplasm was also observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: STAT3 Antibody (9D8) [NBP2-22471] - Analysis of STAT3 using anti-STAT3 (9D8) monoclonal antibody (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing STAT3, at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.
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Immunocytochemistry/Immunofluorescence: NDUFS3 Antibody (JU10-32) [NBP2-75587] - Staining NDUFS3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 500 units/mL Recombinant Human IFN‑ alpha (Catalog # 11100-1) for 20 minutes (dark orange filled histogram), then stained with Rabbit Anti-Human/Mouse Phospho‑STAT1 (Y701) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2894) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
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Immunohistochemistry-Paraffin: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Formalin-paraffin human Mantle Cell Lymphoma stained with Cyclin D1 Ab (Clone SPM587).
Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - analysis of Cyclin D1 in human breast cancer cell line MDA-MB-231 cell lysate using anti-Cyclin D1. Image from verified customer review.
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Immunocytochemistry/Immunofluorescence: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY B Raf.
Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Human pancreas tissue using anti-B Raf mouse monoclonal antibody.
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VEGF [HRP]
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[Unconjugated]
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Western blot shows lysates of PC‑12 rat adrenal pheochromocytoma cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, L‑929 mouse fibroblast cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human/Mouse/Rat HTRA2/Omi Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1458) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for HTRA2/Omi at approximately 36 and 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for HTRA2/Omi at approximately 51 kDa (precursor) and 41 kDa (processed) (as indicated) using 2.5 µg/mL of Rabbit Anti-Human/Mouse/Rat HTRA2/Omi Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1458). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
HTRA2/Omi was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Rabbit Anti-Human/Mouse/Rat HTRA2/Omi Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1458) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (yellow; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Applications: WB, Simple Western, ICC
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Control Lysate(s)

HeLa Whole Cell Lysate
GRIM19 Overexpression Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol NDUFA13
Entrez
Uniprot