NDUFS3 Antibody (HL2097)

Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

NDUFS3 antibody [HL2097] detects NDUFS3 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.NDUFS3 stained by NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NDUFS3 antibody [HL2097] detects NDUFS3 protein at mitochondria by immunofluorescent analysis.Sample: 293T cells were fixed in ice-cold MeOH for 5 min.Green: NDUFS3 stained by NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .

Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

NDUFS3 antibody [HL2097] detects NDUFS3 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.NDUFS3 stained by NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole Japanese medaka extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Ze
• Applications: WB, ICC/IF, IHC
• Clone: HL2097
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

NDUFS3 Antibody (HL2097) Summary

• Description: Novus Biologicals Rabbit NDUFS3 Antibody (HL2097) (NBP3-48671) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human NDUFS3
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NDUFS3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for NDUFS3 Antibody (HL2097)

• CI-30
• CI-30kD
• complex I 30kDa subunit
• Complex I-30kD
• EC 1.6.5.3
• EC 1.6.99.3
• EC 1.6.99.5
• NADH dehydrogenase (ubiquinone) Fe-S protein 3 (30kD) (NADH-coenzyme Qreductase)
• NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Qreductase)
• NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
• NADH dehydrogenase-ubiquinone 30 kDa subunit
• NADH-ubiquinone oxidoreductase 30 kDa subunit

Background

The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits. See NDUFS1 (MIM 157655).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFS3