GRG (Groucho homolog) Antibody

Images

 
Western Blot: GRG (Groucho homolog) Antibody [NBP1-76645] - Analysis of GRG (Groucho homolog) in 293 cell lysate with GRG (Groucho homolog) antibody using NBP1-76645 at (A) 1, (B) 2 and (C) 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: GRG (Groucho homolog) Antibody [NBP1-76645] - Immunocytochemistry of GRG (Groucho homolog) in HeLa cells using NBP1-76645 at 10 ug/ml.
Immunocytochemistry/ Immunofluorescence: GRG (Groucho homolog) Antibody [NBP1-76645] - in Hela cells

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GRG (Groucho homolog) Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human AES. The immunogen is located within the last 50 amino acids of AES.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLE5
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-4 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
Control
GRG (Groucho homolog) Knockout HeLa Cell Lysate
Control Peptide
GRG (Groucho homolog) Peptide (NBP1-76645PEP)
Publications
Read Publications using
NBP1-76645 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for GRG (Groucho homolog) Antibody

  • AES-1
  • AES-2
  • Amino enhancer of split
  • amino-terminal enhancer of split
  • ESP1
  • Gp130-associated protein GAM
  • GRG
  • GRG5
  • Protein ESP1
  • Protein GRG
  • TLE5

Background

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-46134
Species: Hu
Applications: WB, Flow, IHC, IHC-P, IF
NBP2-58917
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP1-86615
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-44520
Species: Hu, Ca(-), Rt(-)
Applications: WB, Flow, IHC, IHC-P, Flow-IC, IF
NBP2-15140
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87581
Species: Hu
Applications: WB, IHC, IHC-P
H00009700-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-20053
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-56603
Species: Hu, Mu, Pm
Applications: WB, IHC, IHC-P
NBP1-82845
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-38530
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89146
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-01733
Species: Hu
Applications: WB, Flow, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-79843
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, CyTOF-ready
NBP1-83906
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-32244
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for GRG (Groucho homolog) Antibody (NBP1-76645)(3)

Reviews for GRG (Groucho homolog) Antibody (NBP1-76645) (0)

There are no reviews for GRG (Groucho homolog) Antibody (NBP1-76645). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GRG (Groucho homolog) Antibody (NBP1-76645) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional GRG (Groucho homolog) Products

Bioinformatics Tool for GRG (Groucho homolog) Antibody (NBP1-76645)

Discover related pathways, diseases and genes to GRG (Groucho homolog) Antibody (NBP1-76645). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GRG (Groucho homolog) Antibody (NBP1-76645)

Discover more about diseases related to GRG (Groucho homolog) Antibody (NBP1-76645).
 

Pathways for GRG (Groucho homolog) Antibody (NBP1-76645)

View related products by pathway.

PTMs for GRG (Groucho homolog) Antibody (NBP1-76645)

Learn more about PTMs related to GRG (Groucho homolog) Antibody (NBP1-76645).
 

Research Areas for GRG (Groucho homolog) Antibody (NBP1-76645)

Find related products by research area.

Blogs on GRG (Groucho homolog)

There are no specific blogs for GRG (Groucho homolog), but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GRG (Groucho homolog) Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol TLE5
Entrez
Uniprot