GRG (Groucho homolog) Antibody - BSA Free

Western Blot: GRG (Groucho homolog) Antibody [NBP1-76645] - Analysis of GRG (Groucho homolog) in 293 cell lysate with GRG (Groucho homolog) antibody using NBP1-76645 at (A) 1, (B) 2 and (C) 4 ug/ml.

Immunocytochemistry/ Immunofluorescence: GRG (Groucho homolog) Antibody - BSA Free [NBP1-76645] - Immunofluorescence of GRG (Groucho homolog) in Hela cells with GRG (Groucho homolog) antibody at 20 ug/mL.

Immunocytochemistry/ Immunofluorescence: GRG (Groucho homolog) Antibody - BSA Free [NBP1-76645] - Immunocytochemistry of GRG (Groucho homolog) in HeLa cells with GRG (Groucho homolog) antibody at 10 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GRG (Groucho homolog) Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GRG (Groucho homolog) Antibody - BSA Free (NBP1-76645) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-GRG (Groucho homolog) Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human AES. The immunogen is located within the last 50 amino acids of AES. Amino Acid Squence: GHDGDTHQEDDGEKSD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TLE5
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/ml
  • Western Blot 1-4 ug/ml
• Control Peptide:

  GRG (Groucho homolog) Peptide (NBP1-76645PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for GRG (Groucho homolog) Antibody - BSA Free

• AES-1
• AES-2
• Amino enhancer of split
• amino-terminal enhancer of split
• ESP1
• Gp130-associated protein GAM
• GRG
• GRG5
• Protein ESP1
• Protein GRG
• TLE5

Background

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GRG (Groucho homolog) Antibody (NBP1-76645)(3)

Publications using NBP1-76645

• Ogorodnikov A, Levin M, Tattikota S et al. Transcriptome 3'end organization by PCF11 links alternative polyadenylation to formation and neuronal differentiation of neuroblastoma Nat Commun 2018-12-14 [PMID: 30552333] (WB, Human)
• Steffen B, Knop M, Bergholz U et al. AML1/ETO induces self-renewal in hematopoietic progenitor cells via the Groucho-related amino-terminal AES protein. Blood. 2011-04-21 [PMID: 21245488] (ICC/IF, WB)
  
  Details:
  Product cited: AES (IMG-5408). 1. IF/ICC: HeLa and suspension HL-60 cells, Fig 1E. 2. WB: U937 cells transfected with AML1/ETO vector, Fig 1B.
• Owen LA, Kowalewski AA, Lessnick SL. EWS/FLI mediates transcriptional repression via NKX2.2 during oncogenic transformation in Ewing's sarcoma. PLoS One. 2008-04-16 [PMID: 18414662] (WB)
  
  Details:
  WB: Fig 4A (retroviral-mediated expression of AES and empty vector control in A673 Ewing's sarcoma cells). Note: The specificity of the AES (IMG-5408) antibody was validated using retroviral-mediated AES expression compared to the empty vector control.

Bioinformatics

• Gene Symbol: TLE5
• Entrez:
  • 166(Human)
• Uniprot:
  • Q08117(Human)