GRASP55 Antibody - BSA Free

Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP2-38244] - Immunohistochemistry analysis in human epididymis and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.

Western Blot: GRASP55 Antibody [NBP2-38244] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GORASP2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: GRASP55 Antibody [NBP2-38244] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP2-38244] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP2-38244] - Staining of human cervix, uterine shows moderate positivity in golgi apparatus in squamous epithelial cells.

Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP2-38244] - Staining of human placenta shows moderate positivity in golgi apparatus in trophoblastic cells.

Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP2-38244] - Staining of human prostate shows moderate positivity in golgi apparatus in glandular cells.

Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP2-38244] - Staining of human epididymis shows moderate to strong positivity in golgi apparatus in glandular cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GRASP55 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GRASP55 Antibody - BSA Free (NBP2-38244) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-GRASP55 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: SGELLSSLPPTSNAPSDPATTTAKADAASSLTVDVTPPTAKAPTTVEDRVGDSTPVSEKPVSAAVDANASESP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GORASP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  GRASP55 Recombinant Protein Antigen (NBP2-38244PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for GRASP55 Antibody - BSA Free

• Golgi phosphoprotein 6
• golgi reassembly stacking protein 2, 55 kDa
• golgi reassembly stacking protein 2, 55kDa
• Golgi reassembly-stacking protein of 55 kDa
• GOLPH6DKFZp434D156
• GRASP55FLJ13139
• GRS2Golgi reassembly-stacking protein 2
• p59

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in establishing the stacked structure of the Golgi apparatus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GRASP55 Antibody (NBP2-38244)(1)

Publication using NBP2-38244

• Akihiro Harada, Masataka Kunii, Kazuo Kurokawa, Takuya Sumi, Satoshi Kanda, Yu Zhang, Satomi Nadanaka, Koichiro M. Hirosawa, Kazuaki Tokunaga, Takuro Tojima, Manabu Taniguchi, Kenta Moriwaki, Shin-ichiro Yoshimura, Miki Yamamoto-Hino, Satoshi Goto, Toyomasa Katagiri, Satoshi Kume, Mitsuko Hayashi-Nishino, Miyako Nakano, Eiji Miyoshi, Kenichi G. N. Suzuki, Hiroshi Kitagawa, Akihiko Nakano Dynamic movement of the Golgi unit and its glycosylation enzyme zones Nature Communications 2024-05-27 [PMID: 38802491]

Bioinformatics

• Gene Symbol: GORASP2
• Uniprot:
  • Q9H8Y8(Human)