IFIT1 Antibody (OTI3G8)

Images

 
Western Blot: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IFIT1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: IFIT1 Antibody (3G8) [NBP2-02340] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT1.
Immunohistochemistry-Paraffin: IFIT1 Antibody (3G8) [NBP2-02340] - Staining of paraffin-embedded Human liver tissue using anti-IFIT1 mouse monoclonal antibody.
Flow Cytometry: IFIT1 Antibody (3G8) [NBP2-02340] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IFIT1 antibody, and then analyzed ...read more
Western Blot: IFIT1 Antibody (3G8) [NBP2-02340] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Western Blot: IFIT1 Antibody (3G8) [NBP2-02340] Analysis of extracts (35ug) from 9 different cell lines by using anti-IFIT1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: IFIT1 Antibody (3G8) [NBP2-02340] - Immunofluorescent staining of HT29 cells using anti-IFIT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IFIT1 Antibody (3G8) [NBP2-02340] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IFIT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IFIT1 Antibody (3G8) [NBP2-02340] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-IFIT1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IFIT1 Antibody (3G8) [NBP2-02340] - Staining of paraffin-embedded Human Kidney tissue using anti-IFIT1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI3G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

IFIT1 Antibody (OTI3G8) Summary

Immunogen
Full length recombinant protein of human IFIT1 (NP_000899) produced in HEK293T cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IFIT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Application Notes
For IHC-P, we recommend using Tris/EDTA pH 8.0 heat induced epitope retrieval.
Theoretical MW
55.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IFIT1 Antibody (OTI3G8)

  • G10P1C56
  • GARG-16
  • IFI56IFI-56
  • IFI-56K
  • IFIT-1
  • IFNAI1ISG56
  • Interferon, alpha-inducible protein (MW 56kD)
  • Interferon-induced 56 kDa protein
  • interferon-induced protein 56
  • interferon-induced protein with tetratricopeptide repeats 1
  • p56
  • RNM561

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IFIT1 Antibody (NBP2-02340) (0)

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Secondary Antibodies

 

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Diseases for IFIT1 Antibody (NBP2-02340)

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PTMs for IFIT1 Antibody (NBP2-02340)

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Blogs on IFIT1.

STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also...  Read full blog post.

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Bioinformatics

Gene Symbol IFIT1
Entrez