Grancalcin Antibody (2F5) - Azide and BSA Free

Western Blot: Grancalcin Antibody (2F5) [H00025801-M01] - GCA monoclonal antibody (M01), clone 2F5 Analysis of GCA expression in MCF-7.

Immunocytochemistry/Immunofluorescence: Grancalcin Antibody (2F5) [H00025801-M01] - Analysis of monoclonal antibody to GCA on MCF-7 cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: Grancalcin Antibody (2F5) [H00025801-M01] - Detection limit for recombinant GST tagged GCA is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Grancalcin Antibody (2F5) - Azide and BSA Free Summary

• Immunogen: GCA (AAH05214, 1 a.a. ~ 217 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAI
• Specificity: GCA (2F5)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GCA
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Grancalcin Antibody (2F5) - Azide and BSA Free

• EF-hand calcium-binding protein
• grancalcin, EF-hand calcium binding protein
• grancalcin, penta-EF-hand protein

Background

This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Grancalcin Antibody (H00025801-M01)(1)

Publication using H00025801-M01

• Schoolmeesters A, Eklund T, Leake D et al. Functional profiling reveals critical role for miRNA in differentiation of human mesenchymal stem cells. PLoS One 2009-05-19 [PMID: 19440384]

Bioinformatics

• Gene Symbol: GCA
• Entrez:
  • 25801(Human)
• OMIM:
  • 607030(Human)
• Uniprot:
  • AAH05214(Human)