GPD2 Antibody - BSA Free

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining in human parathyroid gland and liver tissues using anti-GPD2 antibody. Corresponding GPD2 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human colon, liver, parathyroid gland and skin using Anti-GPD2 antibody NBP2-38542 (A) shows similar protein distribution across tissues to independent antibody NBP1-86121 (B).

Western Blot: GPD2 Antibody [NBP2-38542] - Analysis using Anti-GPD2 antibody NBP2-38542 (A) shows similar pattern to independent antibody NBP1-86121 (B).

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human parathyroid gland shows high expression.

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human liver shows low expression as expected.

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human colon.

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human skin.

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human liver using Anti-GPD2 antibody NBP2-38542.

Immunohistochemistry-Paraffin: GPD2 Antibody [NBP2-38542] - Staining of human parathyroid gland using Anti-GPD2 antibody NBP2-38542.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GPD2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GPD2 Antibody - BSA Free (NBP2-38542) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: TAVDMISRRTRLAFLNVQAAEEALPRIVELMGRELNWDDYKKQEQLETARKFLYYEMGYKSRSEQLTDRSEISLLPSDIDRYKKRFHKFDADQKGFITIVDVQRVLESINVQMDENTL
• Predicted Species: Mouse (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPD2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  GPD2 Protein (NBP2-38542PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for GPD2 Antibody - BSA Free

• EC 1.1.5.3
• GDH2
• glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
• GPD2
• GPDH-M
• GPDM
• GPD-M
• mGDH
• mGPDH
• mitochondrial glycerophosphate dehydrogenase
• mitochondrial
• mtGPD

Background

Mitochondrial glycerophosphate dehydrogenase (EC 1.1.99.5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPD2
• Uniprot:
  • P43304(Human)