Glycophorin C Antibody (9K8U2)

Western Blot: Glycophorin C Antibody (9K8U2) [NBP3-15385] - Western blot analysis of extracts of various cell lines, using Glycophorin C (GYPC) Rabbit mAb (NBP3-15385) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry-Paraffin: Glycophorin C Antibody (9K8U2) [NBP3-15385] - Immunohistochemistry of paraffin-embedded human placenta using Glycophorin C (GYPC) Rabbit mAb (NBP3-15385) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry: Glycophorin C Antibody (9K8U2) [Glycophorin C] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Glycophorin C Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Western Blot: Glycophorin C Antibody (9K8U2) [Glycophorin C] - Western blot analysis of various lysates using Glycophorin C Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

Immunohistochemistry: Glycophorin C Antibody (9K8U2) [Glycophorin C] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using Glycophorin C Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Western Blot: Glycophorin C Antibody (9K8U2) [Glycophorin C] - Western blot analysis of lysates from K-562 cells, using Glycophorin C Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

Immunohistochemistry: Glycophorin C Antibody (9K8U2) [Glycophorin C] - Immunohistochemistry analysis of paraffin-embedded Human appendix tissue using Glycophorin C Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Immunohistochemistry: Glycophorin C Antibody (9K8U2) [Glycophorin C] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Glycophorin C Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Immunohistochemistry: Glycophorin C Antibody (9K8U2) [Glycophorin C] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Glycophorin C Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 9K8U2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Glycophorin C Antibody (9K8U2) Summary

• Description: Novus Biologicals Rabbit Glycophorin C Antibody (9K8U2) (NBP3-15385) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 49-128 of human Glycophorin C (P04921). METSTPTIMDIVVIAGVIAAVAIVLVSLLFVMLRYMYRHKGTYHTNEAKGTEFAESADAALQGDPALQDAGDSSRKEYFI
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GYPC
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Glycophorin C Antibody (9K8U2)

• CD236 antigen
• CD236
• CD236R
• GE
• GLPC
• glycoconnectin
• glycophorin C (Gerbich blood group)
• glycophorin-C
• glycophorin-D
• Glycoprotein beta
• GPCMGC126191
• GPD
• GYPD
• MGC117309
• MGC126192
• PAS-2
• PAS-2'
• Sialoglycoprotein D

Background

Glycophorin C (GYPC) is an integral membrane glycoprotein. It is a minor species carried by human erythrocytes, butplays an important role in regulating the mechanical stability of red cells. A number of glycophorin C mutations havebeen described. The Gerbich and Yus phenotypes are due to deletion of exon 3 and 2, respectively. The Webb and Duchantigens, also known as glycophorin D, result from single point mutations of the glycophorin C gene. The glycophorin Cprotein has very little homology with glycophorins A and B. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GYPC