GPD2 Antibody (PSH0-31)

Western Blot: GPD2 Antibody (PSH0-31) [NBP3-32402] - Western blot analysis of GPD2 on different lysates with Rabbit anti-GPD2 antibody (NBP3-32402) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: THP-1 cell lysate Lane 3: U87-MG cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 81 kDa Observed band size: 68 kDa Exposure time: 10s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32402) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: GPD2 Antibody (PSH0-31) [NBP3-32402] - Flow cytometric analysis of Hela cells labeling GPD2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32402, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu
• Applications: WB, Flow
• Clone: PSH0-31
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GPD2 Antibody (PSH0-31) Summary

• Description: Novus Biologicals Rabbit GPD2 Antibody (PSH0-31) (NBP3-32402) is a recombinant monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human GPD2 aa 43-727. (Uniprot: P43304)
• Localization: Mitochondrion.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GPD2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Western Blot 1:1000
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GPD2 Antibody (PSH0-31)

• EC 1.1.5.3
• GDH2
• glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
• GPD2
• GPDH-M
• GPDM
• GPD-M
• mGDH
• mGPDH
• mitochondrial glycerophosphate dehydrogenase
• mitochondrial
• mtGPD

Background

Mitochondrial glycerophosphate dehydrogenase (EC 1.1.99.5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPD2