Golgin 97 Antibody

Images

 
Western Blot: Golgin 97 Antibody [NBP2-38528] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver ...read more
Immunocytochemistry/ Immunofluorescence: Golgin 97 Antibody [NBP2-38528] - Immunofluorescent staining of human cell line A549 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Golgin 97 Antibody [NBP2-38528] - Staining in human testis and pancreas tissues using anti-GOLGA1 antibody. Corresponding GOLGA1 RNA-seq data are presented ...read more
Immunohistochemistry-Paraffin: Golgin 97 Antibody [NBP2-38528] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: Golgin 97 Antibody [NBP2-38528] - Staining of human testis shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Golgin 97 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: TGAESKITALEQKEQELQALIQQLSIDLQKVTAETQEKEDVITHLQEKVASLEKRLEQNLSGEEHLQELLK
Specificity
Specificity of human Golgin 97 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GOLGA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Golgin 97 Protein (NBP2-38528PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (85%), Rat (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Golgin 97 Antibody

  • gap junction protein, alpha 4, 37kD
  • golgi autoantigen, golgin subfamily a, 1
  • golgin A1
  • Golgin subfamily A member 1
  • Golgin-97
  • MGC33154

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GOLGA1
Uniprot