Giantin Antibodies
We offer GOLGB1/Giantin Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each GOLGB1/Giantin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our GOLGB1/Giantin Antibodies can be used in a variety of model species: Human, Rat. Use the list below to choose the GOLGB1/Giantin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our GOLGB1/Giantin polyclonal antibodies and browse our GOLGB1/Giantin monoclonal antibody catalog.
Alternate Names for Giantin Antibodies
anti-GOLGB1/Giantin antibody, anti-GOLGB1 antibody, anti-372 kDa Golgi complex-associated protein antibody, anti-GCP antibody, anti-GCP372GIANTIN antibody, anti-Giantin antibody, anti-golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal)1,macrogolgin antibody, anti-golgi integral membrane protein 1 antibody, anti-golgin B1 antibody, anti-golgin B1, golgi integral membrane protein antibody, anti-Golgin subfamily B member 1 antibody, anti-GOLIM1 antibody, anti-Macrogolgin antibody
11 Results for "golgb1-giantin" in Primary Antibodies
![Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - HeLa cell lysate sample treated with DMSO was run on 8% SDS-PAGE. Both giantin monomer and dimer are indicated. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Western-Blot-NBP2-22321-img0010.jpg "Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - HeLa cell lysate sample treated with DMSO was run on 8% SDS-PAGE. Both giantin monomer and dimer are indicated. Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - PC-3 human prostate cancer cell line. Image was captured using EVOS M5000 microscope. Red - giantin; blue - nucleus, DAPI; bar 40 micron. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22321-img0011.jpg)
![Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22321 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Western-Blot-NBP2-22321-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Untreated MDA-MB-231 cells showed perinuclear colonization of Giantin and other proteins, demonstrating this cell line exhibits the architecture of a typical Golgi apparatus. Cells were fixed, permeabilized, and immunolabeled with rabbit polyclonal antibody to Giantin. Secondary antibodies in other channels. Nuclei were stained with DAPI (gray channel). Fluorescence microscopy was used to examine stained cells. The final row is a merged image form the previous 3 rows. Overlapping of different combinations of rows indicated in different colors. Bar: 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29614107/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunofluorescence-NBP2-22321-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Effect of ActD on the perinuclear localization of the Golgi apparatus of MDA-MB-231 cells assessed by immunofluorescence to Giantin, GM130 and TGN46. Cells were fixed, permeabilized, and immunolabeled with rabbit polyclonal antibody to Giantin. The final row is a merged image form the previous 3 rows. Overlapping of different combinations of rows indicated in different colors. Bar: 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29614107/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunofluorescence-NBP2-22321-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP2-22321] - Shows the combination effects of antitumor drugs and ARF1 disruptors on the MDA-MB-231 cells Golgi apparatus. Cells were fixed, permeabilized, and immunolabeled with rabbit polyclonal antibody to Giantin. The final row is a merged image form the previous 3 rows. Overlapping of different combinations of rows indicated in different colors. Bar: 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29614107/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunofluorescence-NBP2-22321-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22321 used for IP at 6 ug/mg lysate. GOLGB1 was also immunoprecipitated by rabbit anti-GOLGB1 antibodies NBP2-22322 and NBP2-22323. For blotting immunoprecipitated GOLGB1, NBP2-22321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunoprecipitation-NBP2-22321-img0003.jpg)
![Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91937] - Staining of human endometrium, gastrointestinal, kidney and prostate using Anti-GOLGB1/Giantin antibody NBP1-91937 (A) shows similar protein distribution across tissues to independent antibody NBP1-91938 (B).](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunohistochemistry-Paraffin-NBP1-91937-img0011.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91937] - Staining of human endometrium, gastrointestinal, kidney and prostate using Anti-GOLGB1/Giantin antibody NBP1-91937 (A) shows similar protein distribution across tissues to independent antibody NBP1-91938 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP1-91937] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91937-img0005.jpg)
![Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91937] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunohistochemistry-Paraffin-NBP1-91937-img0007.jpg)
![Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91937] - Staining of human colon.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunohistochemistry-Paraffin-NBP1-91937-img0008.jpg)
![Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91937] - Staining of human kidney.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunohistochemistry-Paraffin-NBP1-91937-img0009.jpg)
![Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91937] - Staining of human epididymis.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunohistochemistry-Paraffin-NBP1-91937-img0010.jpg)
![Western Blot: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Western blot analysis of GOLGB1/Giantin on different lysates with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: Vero cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse lung tissue lysate (40 ug/Lane) Lane 9: Mouse small intestine tissue lysate (40 ug/Lane) Lane 10: Rat lung tissue lysate (40 ug/Lane) Predicted band size: 376 kDa Observed band size: 376 kDa Exposure time: Lane 1-6: 5 seconds; Lane 7-10: 1 minute 2 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32381) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32381_rabbit-golgb1-giantin-mab-psh03-86-278202416331281.jpg "Western Blot: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Western blot analysis of GOLGB1/Giantin on different lysates with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: Vero cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse lung tissue lysate (40 ug/Lane) Lane 9: Mouse small intestine tissue lysate (40 ug/Lane) Lane 10: Rat lung tissue lysate (40 ug/Lane) Predicted band size: 376 kDa Observed band size: 376 kDa Exposure time: Lane 1-6: 5 seconds; Lane 7-10: 1 minute 2 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32381) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32381) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32381_rabbit-golgb1-giantin-mab-psh03-86-298202414161858.jpg)
![Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Immunocytochemistry analysis of HeLa cells labeling GOLGB1/Giantin with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32381_rabbit-golgb1-giantin-mab-psh03-86-2920241114824.jpg)
![Flow Cytometry: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Flow cytometric analysis of HeLa cells labeling GOLGB1/Giantin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32381, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32381_rabbit-golgb1-giantin-mab-psh03-86-39202488463.jpg)
![Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91938] - Staining of human endometrium, gastrointestinal, kidney and prostate using Anti-GOLGB1/Giantin antibody NBP1-91938 (A) shows similar protein distribution across tissues to independent antibody NBP1-91937 (B).](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunohistochemistry-Paraffin-NBP1-91938-img0011.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91938] - Staining of human endometrium, gastrointestinal, kidney and prostate using Anti-GOLGB1/Giantin antibody NBP1-91938 (A) shows similar protein distribution across tissues to independent antibody NBP1-91937 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP1-91938] - Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91938-img0005.jpg)
![Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91938] - Staining of human epididymis.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunohistochemistry-Paraffin-NBP1-91938-img0007.jpg)
![Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91938] - Staining of human colon.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunohistochemistry-Paraffin-NBP1-91938-img0008.jpg)
![Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91938] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunohistochemistry-Paraffin-NBP1-91938-img0009.jpg)
![Immunohistochemistry-Paraffin: GOLGB1/Giantin Antibody [NBP1-91938] - Staining of human kidney.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunohistochemistry-Paraffin-NBP1-91938-img0010.jpg)
![Western Blot: GOLGB1/Giantin Antibody [NBP2-22323] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22323 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Western-Blot-NBP2-22323-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: GOLGB1/Giantin Antibody [NBP2-22323] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22323 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22323] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22323 used for IP at 6 ug/mg lysate. Giantin was also immunoprecipitated by rabbit anti-Giantin antibodies NBP2-22321 and NBP2-22322. For blotting immunoprecipitated Giantin, NBP2-22323 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunoprecipitation-NBP2-22323-img0003.jpg)
![Western Blot: GOLGB1/Giantin Antibody [NBP2-22322] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22322 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Western-Blot-NBP2-22322-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: GOLGB1/Giantin Antibody [NBP2-22322] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22322 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22322] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22322 used for IP at 6 ug/mg lysate. Giantin was also immunoprecipitated by rabbit anti- Giantin antibodies NBP2-22321 and NBP2-22323. For blotting immunoprecipitated Giantin, NBP2-22322 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-Immunoprecipitation-NBP2-22322-img0003.jpg)
![Sandwich ELISA: GOLGB1/Giantin Antibody (6D4) [H00002804-M01] - Detection limit for recombinant GST tagged GOLGB1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GOLGB1-Giantin-Antibody-6D4-Sandwich-ELISA-H00002804-M01-img0002.jpg "Sandwich ELISA: GOLGB1/Giantin Antibody (6D4) [H00002804-M01] - Detection limit for recombinant GST tagged GOLGB1 is approximately 0.03ng/ml as a capture antibody.")