GOLGA3 Antibody - BSA Free

Western Blot: GOLGA3 Antibody [NBP1-91952] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Immunocytochemistry/Immunofluorescence: GOLGA3 Antibody [NBP1-91952] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus.

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in germinal center cells.

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952] - Staining of human colon shows strong cytoplasmic positivity in lymphoid cells.

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952] - Staining of human colon, prostate, testis and tonsil using Anti-GOLGA3 antibody NBP1-91952 (A) shows similar protein distribution across tissues to independent antibody NBP1-91953 (B).

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts.

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GOLGA3 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: STRGTYGILSKTVGTQDTPYMVNGQEIPADTLGQFPSIKDVLQAAAAEHQDQGQEVNGEVRSRRDSICSSVSLESSAAETQEEMLQVLKEKMRLEG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GOLGA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  GOLGA3 Protein (NBP1-91952PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for GOLGA3 Antibody - BSA Free

• GCP170Golgi peripheral membrane protein
• golgi autoantigen, golgin subfamily a, 3
• Golgi complex-associated protein of 170 kD
• Golgi complex-associated protein of 170 kDa
• Golgi membrane associated protein
• golgin A3
• Golgin subfamily A member 3
• golgin-160
• golgin-165
• male enhanced antigen-2
• MEA-2
• SY2/SY10 protein

Background

GOLGA3 is a member of the golgin family of proteins which are localized to the Golgi. It has been postulated to play a role in nuclear transport and Golgi apparatus localization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GOLGA3 Antibody (NBP1-91952)(1)

Publication using NBP1-91952

• Weigand I, Ronchi CL, Vanselow JT et al. PKA C alpha subunit mutation triggers caspase-dependent RII beta subunit degradation via Ser114 phosphorylation Science advances 2021-02-01 [PMID: 33608270] (WB, Human)

Bioinformatics

• Gene Symbol: GOLGA3