CXCL6/GCP-2 Antibody (60910)

Recombinant Human CXCL6/GCP‑2 (Catalog # 333-GC) induces chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, chemotaxis elicited by CXCL6/GCP‑2 is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL6/GCP‑2 Monoclonal Antibody (Catalog # MAB333). The ND50 is typically 8-30 μg/mL.

• Reactivity: Hu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 60910
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCL6/GCP-2 Antibody (60910) Summary

• Immunogen: E. coli-derived recombinant human CXCL6/GCP-2
  Val40-Asn114
  Accession # P80162
• Specificity: Detects human CXCL6/GCP‑2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) NAP-2, rhGRO alpha , rhGRO beta , rhBLC, rhENA-78, recombinant mouse (rm) CXCL6, rmMIP-2, rhIL-8, rhIP-10, rhMIG, rhSDF-1 alpha , and rhSDF-1 beta is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCL6
• Purity: Protein A or G purified from ascites
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 8-30 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human CXCL6/GCP-2 Biotinylated Antibody (Catalog number BAF333)
  Standard: Recombinant Human CXCL6/GCP-2 (Catalog number 333-GC)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from ascites
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL6/GCP-2 Antibody (60910)

• chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2)
• Chemokine alpha 3
• CKA-3Granulocyte chemotactic protein 2
• CXCL6
• GCP-2
• GCP2member 6 (granulocytechemotactic protein 2)
• GCP-2Small-inducible cytokine B6
• Small inducible cytokine subfamily B (Cys-X-Cys), member b

Background

CXCL6, also known as Granulocyte Chemotactic Protein 2 (GCP-2), belongs to the CXC chemokine family. It functions as a neutrophil chemoattractant through interactions with CXCR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL6/GCP-2 Antibody (MAB333)(2)

Publications using MAB333

• Kelvin K Tsai Metronomic chemotherapy prevents therapy-induced stromal activation and induction of tumor-initiating cells J. Exp. Med., 2016-11-23;0(0):. 2016-11-23 [PMID: 27881732] (Neutralization, Human)
• Penninckx F, Ectors N GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokines in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors. Exp. Cell Res., 2005-02-15;303(2):331-42. 2005-02-15 [PMID: 15652347] (IHC-P, Western Blot, Human)

Review for CXCL6/GCP-2 Antibody (MAB333) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Ruhul Choudhury B/N Mouse 01/20/2017

Summary

• Application: Block/Neutralize
• Sample Tested: Uterine endothelial cells conditioned medium
• Species: Mouse

Bioinformatics

• Gene Symbol: CXCL6
• Uniprot:
  • P80162()