GOLGB1/Giantin Antibody (PSH03-86)

Western Blot: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Western blot analysis of GOLGB1/Giantin on different lysates with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: Vero cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: PC-12 cell lysate (20 ug/Lane) Lane 8: Mouse lung tissue lysate (40 ug/Lane) Lane 9: Mouse small intestine tissue lysate (40 ug/Lane) Lane 10: Rat lung tissue lysate (40 ug/Lane) Predicted band size: 376 kDa Observed band size: 376 kDa Exposure time: Lane 1-6: 5 seconds; Lane 7-10: 1 minute 2 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32381) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32381) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Immunocytochemistry analysis of HeLa cells labeling GOLGB1/Giantin with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GOLGB1/Giantin antibody (NBP3-32381) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: GOLGB1/Giantin Antibody (PSH03-86) [NBP3-32381] - Flow cytometric analysis of HeLa cells labeling GOLGB1/Giantin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32381, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: PSH03-86
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GOLGB1/Giantin Antibody (PSH03-86) Summary

• Description: Novus Biologicals Rabbit GOLGB1/Giantin Antibody (PSH03-86) (NBP3-32381) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human GOLGB1/Giantin aa 2,736-3,235 / 3,259. (Uniprot: Q14789)
• Localization: Golgi apparatus membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GOLGB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
• Theoretical MW: 376 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GOLGB1/Giantin Antibody (PSH03-86)

• 372 kDa Golgi complex-associated protein
• GCP
• GCP372
• GCP372GIANTIN
• Giantin
• GOLGB1
• golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal)1,macrogolgin
• golgi integral membrane protein 1
• golgin B1
• golgin B1, golgi integral membrane protein
• Golgin subfamily B member 1
• GOLIM1
• Macrogolgin

Background

The giantin (GOLGB1) gene codes for a golgin subfamily B member 1 protein that exists in isoform 1 at a length of 3,259 amino acids and a mass of 376 kDa or isoform 2 at 3,269 amino acids long and 377 kDA. GOLGB1 is thought to participate in the development of intercisternal cross-bridges of the Golgi complex. Researchers have investigated its role in hypertension, myopathy, hematopoiesis, encephalitis, and arthritis. GOLGB1 is known to interact with other genes such as PFN2, CAV2, USO1, SLC2A3, and GORASP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GOLGB1