Glypican 3 Antibody [Unconjugated]

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Glypican 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2119) ...read more
Glypican 3 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human Glypican 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2119) at 1.7 µg/mL for 3 ...read more
Glypican 3 was detected in immersion fixed paraffin-embedded sections of human breast using 5 µg/mL Sheep Anti-Human Glypican 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2119) ...read more
Glypican 3 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Sheep Anti-Human Glypican 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2119) at 10 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Glypican 3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Glypican 3
Gln25-Val558
Accession # P51654
Specificity
Detects human Glypican 3 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Glypican 3 is observed and less than 5% cross-reactivity with recombinant human (rh) Glypican 2, rhGlypican 5, and rhGlypican 6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
GPC3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 1-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL
Application Notes
Use in IHC-Fr at 1:50 dilution with IF detection for Rat frozen tissues is reported by Grozdanov et al. in PMID 17117158.
Publications
Read Publications using
AF2119 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glypican 3 Antibody [Unconjugated]

  • DGSX
  • Glypican 3
  • glypican proteoglycan 3
  • glypican-3
  • GPC3
  • GTR2-2
  • heparan sulphate proteoglycan
  • Intestinal protein OCI-5
  • MXR7
  • OCI5
  • OCI-5
  • secreted glypican-3
  • SGB
  • SGBS
  • SGBS1SDYS

Background

Glypicans (GPC) are a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1-GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. While glypican proteins do not share strong amino acid sequence identity (they range from 17-63%), the conserved cysteine residues in their CRDs suggests similarity in their three‑dimensional structure (1, 2).

Mutations in GPC3 cause a rare disorder in humans, Simpson-Golabi-Behmel Syndrome, which is characterized by pre and postnatal overgrowth of multiple tissues and organs and an increased risk for developing embryonic tumors (3). These features are also present in the mouse knock-out of GPC3 indicating that GPC3 regulates cell survival and inhibits cell proliferation during development (4). Glypican 3 has been implicated in regulating many different signaling pathways including: IGF, FGF, BMP, and Wnt. An endoproteolytic processing of GPC3 by proprotein convertases is required for the modulation of Wnt signaling (5). Direct interaction with FGF-basic has been observed and is mediated by the heparan sulfate chains (6).

  1. Filmus, J. and S.B. Selleck (2001) J. Clinical Invest. 108:497. 
  2. De Cat, B and G. David (2001) Seminars in Cell & Dev. Biol. 12:117. 
  3. Pilia, G. et al. (1996) Nat. Genet. 12: 241. 
  4. Cano-Gauci, D.F. et al. (1999) J. Cell Biol. 146: 255.
  5. De Cat, B. et al. (2003) J. Cell Biol. 163:625.
  6. Song, H.H. et al. (1997) J. Biol. Chem. 272:7574.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glypican 3 Antibody (AF2119)(5)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 5 applications: ELISA Capture, ELISA Development, IHC-Fr, IHC-P, Western Blot.


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ELISA Capture
(1)
ELISA Development
(1)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(1)
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Human
(3)
Rat
(2)
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Showing Publications 1 - 5 of 5.
Publications using AF2119 Applications Species
N Sun, YT Lee, RY Zhang, R Kao, PC Teng, Y Yang, P Yang, JJ Wang, M Smalley, PJ Chen, M Kim, SJ Chou, L Bao, J Wang, X Zhang, D Qi, J Palomique, N Nissen, SB Han, S Sadeghi, RS Finn, S Saab, RW Busuttil, D Markovic, D Elashoff, HH Yu, H Li, AP Heaney, E Posadas, S You, JD Yang, R Pei, VG Agopian, HR Tseng, Y Zhu Purification of HCC-specific extracellular vesicles on nanosubstrates for early HCC detection by digital scoring Nat Commun, 2020;11(1):4489. 2020 [PMID: 32895384] (ELISA Capture, Human) ELISA Capture Human
YH Khalifa, GM Mourad, WM Stephanos, SA Omar, RA Mehanna Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino Rats Biomed Res Int, 2019;2019(0):5376165. 2019 [PMID: 31781620] (IHC-P, Rat) IHC-P Rat
Phase I trial of a glypican-3-derived peptide vaccine for advanced hepatocellular carcinoma: immunologic evidence and potential for improving overall survival. Clin. Cancer Res., 2012;18(13):3686-96. 2012 [PMID: 22577059] (ELISA Development, Human) ELISA Development Human
Grozdanov PN, Yovchev MI, Dabeva MD The oncofetal protein glypican-3 is a novel marker of hepatic progenitor/oval cells. Lab. Invest., 2006;86(12):1272-84. 2006 [PMID: 17117158] (IHC-Fr, Rat) IHC-Fr Rat
Manton KJ, Sadasivam M, Cool SM, Nurcombe V Bone-specific heparan sulfates induce osteoblast growth arrest and downregulation of retinoblastoma protein. J. Cell. Physiol., 2006;209(1):219-29. 2006 [PMID: 16826571] (Western Blot, Human) Western Blot Human

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Blogs on Glypican 3.

Glypican 3 as a biomarker for gastro-esophageal adenocarcinoma
By Jamshed Arslan, Pharm. D., PhD. Gastroesophageal adenocarcinoma originates from the glandular epithelium of the esophagus, gastroesophageal junction and stomach. The incidence of gastroesophageal adenocarcinoma is ...  Read full blog post.

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Bioinformatics

Gene Symbol GPC3
Uniprot