Glypican 3 Antibody [Unconjugated]


Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Glypican 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2119) more
Glypican 3 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human Glypican 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2119) at 1.7 µg/mL for 3 more
Glypican 3 was detected in immersion fixed paraffin-embedded sections of human breast using 5 µg/mL Sheep Anti-Human Glypican 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2119) more
Glypican 3 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Sheep Anti-Human Glypican 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2119) at 10 µg/mL more
Recombinant Human Glypican 3 protein was serially diluted 2-fold and captured by Mouse Anti-Human Glypican 3 Monoclonal Antibody (MAB21191) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready, ICC/IF

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Glypican 3 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human Glypican 3
Accession # P51654
Detects human Glypican 3 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Glypican 3 is observed and less than 5% cross-reactivity with recombinant human (rh) Glypican 2, rhGlypican 5, and rhGlypican 6 is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 1-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL
Application Notes
Use in IHC-Fr at 1:50 dilution with IF detection for Rat frozen tissues is reported by Grozdanov et al. in PMID 17117158.
Read Publications using
AF2119 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glypican 3 Antibody [Unconjugated]

  • DGSX
  • Glypican 3
  • glypican proteoglycan 3
  • glypican-3
  • GPC3
  • GTR2-2
  • heparan sulphate proteoglycan
  • Intestinal protein OCI-5
  • MXR7
  • OCI5
  • OCI-5
  • secreted glypican-3
  • SGB
  • SGBS


Glypicans (GPC) are a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1-GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. While glypican proteins do not share strong amino acid sequence identity (they range from 17-63%), the conserved cysteine residues in their CRDs suggests similarity in their three‑dimensional structure (1, 2).

Mutations in GPC3 cause a rare disorder in humans, Simpson-Golabi-Behmel Syndrome, which is characterized by pre and postnatal overgrowth of multiple tissues and organs and an increased risk for developing embryonic tumors (3). These features are also present in the mouse knock-out of GPC3 indicating that GPC3 regulates cell survival and inhibits cell proliferation during development (4). Glypican 3 has been implicated in regulating many different signaling pathways including: IGF, FGF, BMP, and Wnt. An endoproteolytic processing of GPC3 by proprotein convertases is required for the modulation of Wnt signaling (5). Direct interaction with FGF-basic has been observed and is mediated by the heparan sulfate chains (6).

  1. Filmus, J. and S.B. Selleck (2001) J. Clinical Invest. 108:497. 
  2. De Cat, B and G. David (2001) Seminars in Cell & Dev. Biol. 12:117. 
  3. Pilia, G. et al. (1996) Nat. Genet. 12: 241. 
  4. Cano-Gauci, D.F. et al. (1999) J. Cell Biol. 146: 255.
  5. De Cat, B. et al. (2003) J. Cell Biol. 163:625.
  6. Song, H.H. et al. (1997) J. Biol. Chem. 272:7574.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glypican 3 Antibody (AF2119)(6)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 6 applications: ELISA Capture, ELISA Development, Flow Cytometry, IHC-Fr, IHC-P, Western Blot.

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ELISA Development
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Showing Publications 1 - 6 of 6.
Publications using AF2119 Applications Species
N Sun, YT Lee, RY Zhang, R Kao, PC Teng, Y Yang, P Yang, JJ Wang, M Smalley, PJ Chen, M Kim, SJ Chou, L Bao, J Wang, X Zhang, D Qi, J Palomique, N Nissen, SB Han, S Sadeghi, RS Finn, S Saab, RW Busuttil, D Markovic, D Elashoff, HH Yu, H Li, AP Heaney, E Posadas, S You, JD Yang, R Pei, VG Agopian, HR Tseng, Y Zhu Purification of HCC-specific extracellular vesicles on nanosubstrates for early HCC detection by digital scoring Nat Commun, 2020-09-07;11(1):4489. 2020-09-07 [PMID: 32895384] (ELISA Capture, Human) ELISA Capture Human
YH Khalifa, GM Mourad, WM Stephanos, SA Omar, RA Mehanna Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino Rats Biomed Res Int, 2019-11-06;2019(0):5376165. 2019-11-06 [PMID: 31781620] (IHC-P, Rat) IHC-P Rat
M Rahbari, M Pecqueux, D Aust, H Stephan, O Tiebel, A Chatzigeor, T Tonn, F Baenke, V Rao, N Ziegler, H Greif, K Lin, J Weitz, NN Rahbari, C Kahlert Expression of Glypican 3 is an Independent Prognostic Biomarker in Primary Gastro-Esophageal Adenocarcinoma and Corresponding Serum Exosomes J Clin Med, 2019-05-16;8(5):. 2019-05-16 [PMID: 31100935] (Flow Cytometry, Western Blot, Human) Flow Cytometry, Western Blot Human
Phase I trial of a glypican-3-derived peptide vaccine for advanced hepatocellular carcinoma: immunologic evidence and potential for improving overall survival. Clin. Cancer Res., 2012-05-10;18(13):3686-96. 2012-05-10 [PMID: 22577059] (ELISA Development, Human) ELISA Development Human
Grozdanov PN, Yovchev MI, Dabeva MD The oncofetal protein glypican-3 is a novel marker of hepatic progenitor/oval cells. Lab. Invest., 2006-10-02;86(12):1272-84. 2006-10-02 [PMID: 17117158] (IHC-Fr, Rat) IHC-Fr Rat
Manton KJ, Sadasivam M, Cool SM, Nurcombe V Bone-specific heparan sulfates induce osteoblast growth arrest and downregulation of retinoblastoma protein. J. Cell. Physiol., 2006-10-01;209(1):219-29. 2006-10-01 [PMID: 16826571] (Western Blot, Human) Western Blot Human

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Blogs on Glypican 3.

Glypican 3 as a biomarker for gastro-esophageal adenocarcinoma
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Gene Symbol GPC3