Glypican 1 Antibody

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Biological Strategies: Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ...read more
Immunocytochemistry/ Immunofluorescence: Glypican 1 Antibody [NBP1-33197] - MCF7 cells were fixed in ice-cold MeOH for 5 min. Green: Glypican 1 protein stained by Glypican 1 antibody [N3C3] diluted at 1:400. Blue: ...read more
Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP1-33197] - Analysis of paraffin-embedded human normal pancreas (left) or pancreatic adenocarcinoma (grade II) (right) tissues using Glypican-1 antibody [N3C3] at a ...read more
Flow Cytometry: Glypican 1 Antibody [NBP1-33197] - Sample: A431 cell. Black: Unlabelled sample was used as a control. Red: Glypican 1 antibody [N3C3]dilution: 1:100. Acquisition of 20,000 events were collected using a ...read more
Biological Strategies: Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 ...read more
Genetic Strategies: Western Blot: Glypican 1 Antibody [NBP1-33197] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ...read more
Western Blot: Glypican 1 Antibody [NBP1-33197] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500.The observed M.W. is ...read more
Western Blot: Glypican 1 Antibody [NBP1-33197] - Mouse tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500.
Biological Strategies: Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 ...read more
Western Blot: Glypican 1 Antibody [NBP1-33197] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500. ...read more
Western Blot: Glypican 1 Antibody [NBP1-33197] - U87-MG whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500. The ...read more
ELISA: Glypican 1 Antibody [NBP1-33197] - An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 antibody at concentration ranged from 7.5 to 240 ng/mL.
Immunoprecipitation: Glypican 1 Antibody [NBP1-33197] - Immunoprecipitation of Glypican 1 protein from MCF-7 whole cell extracts using 5 ug of Glypican 1 antibody [N3C3].
Immunoprecipitation: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] ...read more

Product Details

Summary
Reactivity Hu, Mu, RMSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Glypican 1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.
Localization
Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side, Secreted glypican-1: Secreted, extracellular space
Predicted Species
Rhesus Macaque (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • ELISA 1:1000-1:10000
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rat (88%), Bovine (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Glypican 1 Antibody

  • FLJ38078
  • Glypican 1
  • glypican proteoglycan 1
  • glypican
  • glypican-1
  • GPC1

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GPC1