Novus Biologicals products are now on bio-techne.com

Glypican 1 Antibody

Images

 
Western Blot: Glypican 1 Antibody [NBP1-33197] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody [N3C3] diluted at 1:500 and competitor's ...read more
Immunocytochemistry/ Immunofluorescence: Glypican 1 Antibody [NBP1-33197] - Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: MCF-7 cells were ...read more
Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP1-33197] - Paraffin-embedded human normal pancreas (left) and pancreatic cancer (right).Glypican 1 stained by Glypican 1 antibody [N3C3] diluted at 1:500.Antigen ...read more
Flow Cytometry: Glypican 1 Antibody [NBP1-33197] - Sample: A431 cell. Black: Unlabelled sample was used as a control. Red: Glypican 1 antibody [N3C3]dilution: 1:100. Acquisition of 20,000 events were collected using a ...read more
Biological Strategies: Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 ...read more
Genetic Strategies: Western Blot: Glypican 1 Antibody [NBP1-33197] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ...read more
Western Blot: Glypican 1 Antibody [NBP1-33197] - Mouse tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500.
Biological Strategies: Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 ...read more
Western Blot: Glypican 1 Antibody [NBP1-33197] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500. ...read more
Western Blot: Glypican 1 Antibody [NBP1-33197] - U87-MG whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500. The ...read more
Biological Strategies: Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ...read more
ELISA: Glypican 1 Antibody [NBP1-33197] - An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 antibody at concentration ranged from 7.5 to 240 ng/mL.
Immunoprecipitation: Glypican 1 Antibody [NBP1-33197] - Immunoprecipitation of Glypican 1 protein from MCF-7 whole cell extracts using 5 ug of Glypican 1 antibody [N3C3].
Immunoprecipitation: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RMSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Glypican 1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.
Localization
Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side, Secreted glypican-1: Secreted, extracellular space
Predicted Species
Rhesus Macaque (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:1000-1:10000
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-33197 in the following applications:

  • WB
    1 publication

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Glypican 1 Antibody

  • FLJ38078
  • Glypican 1
  • glypican proteoglycan 1
  • glypican
  • glypican-1
  • GPC1

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF2780
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, WB
BBA10
Species: Hu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
AF6585
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
233-FB
Species: Hu
Applications: BA
AF2119
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC, IHC, WB
AF2364
Species: Hu
Applications: IHC, WB
NBP2-23603
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-02541
Species: Hu, Pm, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-24653
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
AF143
Species: Hu
Applications: IHC, Simple Western, WB
NBP2-16471
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-87697
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-93577
Species: Hu, Mu
Applications: ICC/IF, WB
H00002523-P01
Species: Hu
Applications: ELISA, AP, PA, WB
AF3054
Species: Hu
Applications: ICC, IHC, WB
H00003077-M01
Species: Hu
Applications: ELISA, ICC/IF, WB
NBP1-91258
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP1-33197
Species: Hu, Mu, Rt, RM
Applications: WB, ELISA, Flow, ICC/IF, IHC, IP

Publications for Glypican 1 Antibody (NBP1-33197)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for Glypican 1 Antibody (NBP1-33197) (0)

There are no reviews for Glypican 1 Antibody (NBP1-33197). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Glypican 1 Antibody (NBP1-33197) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Glypican 1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol GPC1