Recombinant E. coli Glutaminase Protein

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SDS-Page: Recombinant E. coli Glutaminase Protein [NBP1-72301] - 15% SDS-Page (3ug)

Product Details

Summary
Reactivity EcSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant E. coli Glutaminase Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-310 of E.coli Glutaminase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLDANKLQQA VDQAYTQFHS LNGGQNADYI PFLANVPGQL AAVAIVTCDG NVYSAGDSDY RFALESISKV CTLALALEDV GPQAVQDKIG ADPTGLPFNS VIALELHGGK PLSPLVNAGA IATTSLINAE NVEQRWQRIL HIQQQLAGEQ VALSDEVNQS EQTTNFHNRA IAWLLYSAGY LYCDAMEACD VYTRQCSTLL NTIELATLGA TLAAGGVNPL THKRVLQADN VPYILAEMMM EGLYGRSGDW AYRVGLPGKS GVGGGILAVV PGVMGIAAFS PPLDEDGNSV RGQKMVASVA KQLGYNVFKG

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GLS
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant E. coli Glutaminase Protein

  • AAD20
  • EC 3.5.1.2
  • GLS1DKFZp686O15119
  • glutaminase C
  • glutaminase kidney isoform, mitochondrial
  • glutaminase
  • glutaminase, phosphate-activated
  • K-glutaminase
  • KIAA0838FLJ10358
  • L-glutamine amidohydrolase

Background

GlsA1 (glutaminase1) belongs to the large superfamily of serine-dependent beta-lactamases and penicillin-binding proteins. They are present in most bacteria and eukaryotes and catalyze the hydrolytic deamidation of l-glutamine to l-glutamate and free NH4+. Recombinant E.coli glsA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol GLS