Glutaminase Antibody

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Western Blot: Glutaminase Antibody [NBP2-29940] - Western blot analysis of lysates from human brain tissue and Hela cell line (from left to right), using (C-term)(Cat. # NBP2-29940). The antibody was diluted at 1:1000 ...read more
Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody [NBP2-29940] - Fluorescent image of Hela cells stained with XAF1 (C-term)(Cat# NBP2-29940). The antibody was diluted at 1:25 dilution. An Alexa Fluor ...read more
Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP2-29940] - Immunohistochemical analysis of paraffin-embedded H.kidney section using (C-term)(Cat# NBP2-29940). The antibody was diluted at 1:25 dilution. A ...read more
Flow Cytometry: Glutaminase Antibody [NBP2-29940] - flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies ...read more
Western Blot: Glutaminase Antibody [NBP2-29940] - Western blot analysis of (C-term) (NBP2-29940) in mouse liver tissue lysates (35ug/lane). Glutaminase (arrow) was detected using the purified Pab.
Western Blot: Glutaminase Antibody [NBP2-29940] - Western blot analysis of lysates from 293, 293T, Hela cell line, human brain and mouse brain tissue lysate (from left to right), using (C-term)(Cat. # NBP2-29940). The ...read more
Western Blot: Glutaminase Antibody [NBP2-29940] - Western blot analysis of lysate from Hela cell line, using Glutaminase Antibody (C-term)(Cat. # NBP2-29940). The antibody was diluted at 1:1000. A goat anti-rabbit IgG ...read more
Western Blot: Glutaminase Antibody [NBP2-29940] - Western blot analysis of lysates from 293T, Hela cell line (from left to right), using Glutaminase Antibody (C-term)(Cat. # NBP2-29940). The antibody was diluted at ...read more
Western Blot: Glutaminase Antibody [NBP2-29940] - Western blot analysis of lysates from human brain, mouse brain ad rat kidney tissue lysate (from left to right), using (C-term)(Cat. # NBP2-29940). The antibody was ...read more
Western Blot: Glutaminase Antibody [NBP2-29940] - Western blot analysis of lysates from human brain and mouse brain tissue lysate (from left to right), using (C-term)(Cat. # NBP2-29940). The antibody was diluted at ...read more
Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody [NBP2-29940] - Confocal immunofluorescent analysis of (C-term) (NBP2-29940) with Hela cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG ...read more
Immunocytochemistry/ Immunofluorescence: Glutaminase Antibody [NBP2-29940] - Fluorescent image of Hela cells stained with XAF1 (C-term)(Cat# NBP2-29940). The antibody was diluted at 1:25 dilution. An Alexa Fluor ...read more
Immunohistochemistry-Paraffin: Glutaminase Antibody [NBP2-29940] - IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.34 mg/ml

Order Details

Glutaminase Antibody Summary

Immunogen
This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.
Localization
Cellular Location Isoform 1: Cytoplasm, cytosol. Tissue Location Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver Isoform 2 is expressed in cardiac and skeletal muscle
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLS
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:25
  • Immunocytochemistry/Immunofluorescence 1:25
  • Immunohistochemistry-Paraffin 1:25
Theoretical MW
73.461 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-29940 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
0.34 mg/ml
Purity
Protein A purified

Alternate Names for Glutaminase Antibody

  • AAD20
  • EC 3.5.1.2
  • GLS1DKFZp686O15119
  • glutaminase C
  • glutaminase kidney isoform, mitochondrial
  • glutaminase
  • glutaminase, phosphate-activated
  • K-glutaminase
  • KIAA0838FLJ10358
  • L-glutamine amidohydrolase

Background

Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glutaminase Antibody (NBP2-29940)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GLS
Uniprot