Glutaminase Antibody (RB22875)

Product Discontinued

Glutaminase Antibody (RB22875) Summary

• Immunogen: This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.
• Localization: Cellular Location Isoform 1: Cytoplasm, cytosol. Tissue Location Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver Isoform 2 is expressed in cardiac and skeletal muscle
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:25
  • Immunocytochemistry/ Immunofluorescence 1:25
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:25
  • Western Blot 1:1000
• Theoretical MW: 73.461 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Porcine reactivity reported by customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.34 mg/ml
• Purity: Protein A purified

Alternate Names for Glutaminase Antibody (RB22875)

• AAD20
• EC 3.5.1.2
• GLS
• GLS1DKFZp686O15119
• glutaminase C
• glutaminase kidney isoform, mitochondrial
• Glutaminase
• glutaminase, phosphate-activated
• K-Glutaminase
• KIAA0838FLJ10358
• L-Glutamine Amidohydrolase

Background

Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutaminase Antibody (NBP2-29940)(5)

Publications using NBP2-29940

• Dorai T, Dorai B, Pinto JT et al. High Levels of Glutaminase II Pathway Enzymes in Normal and Cancerous Prostate Suggest a Role in 'Glutamine Addiction' Biomolecules 2019-12-18 [PMID: 31861280] (WB, Human)
• Dorai T, Shah A, Summers F et al. NRH:quinone oxidoreductase 2 (NQO2) and glutaminase (GLS) both play a role in large extracellular vesicles (LEV) formation in preclinical LNCaP-C4-2B prostate cancer model of progressive metastasis. Prostate. 2018-07-15 [PMID: 30009389] (ICC/IF, Human)
• Pacheco-Velazquez SC, Robledo-Cadena DX, Hernandez-Resendiz I et al. Energy Metabolism Drugs Block Triple Negative Breast Metastatic Cancer Cell Phenotype Mol. Pharm. 2018-05-23 [PMID: 29746779] (Human)
• Chatterjee S, Daenthanasanmak A, Chakraborty P et al. CD38-NAD+Axis Regulates Immunotherapeutic Anti-Tumor T Cell Response. Cell Metab. 2018-01-09 [PMID: 29129787]
  
  Details:
  Citation used the Alexa Fluor 647 format of this antibody.
• Li X, Han G, Li X et al. Mitochondrial pyruvate carrier function determines cell stemness and metabolic reprogramming in cancer cells Oncotarget 2017-07-11 [PMID: 28624784] (WB, Mouse)

Review for Glutaminase Antibody (NBP2-29940) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Pig.

reviewed by: Paula Chen ICC Pig 01/09/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: Porcine embryo
• Species: Pig
• Lot: SA140418AD

Comments

• Comments: Day 6 porcine blastocysts were fixed in 4% paraformaldehyde, permeabilized in 0.1% Triton X-100, and blocked in 2% goat serum. Fixed embryos were incubated in 1:50 anti-glutaminase primary antibody in TL-HEPES overnight at 4 degrees Celsius. The next day, embryos were washed and incubated in 1:500 anti-rabbit IgG with TRITC and imaged.

Bioinformatics

• Gene Symbol: GLS
• Uniprot:
  • O94925(Human)