Glutaminase Antibody (SN68-09)

Western Blot: Glutaminase Antibody (SN68-09) [NBP2-67683] - Analysis of Glutaminase on different lysates using anti-Glutaminase antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293

Immunocytochemistry/Immunofluorescence: Glutaminase Antibody (SN68-09) [NBP2-67683] - Staining Glutaminase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glutaminase Antibody (SN68-09) [NBP2-67683] - Analysis of paraffin-embedded human tonsil tissue using anti-Glutaminase antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: Glutaminase Antibody (SN68-09) [NBP2-67683] - Staining Glutaminase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glutaminase Antibody (SN68-09) [NBP2-67683] - Analysis of paraffin-embedded human kidney tissue using anti-Glutaminase antibody. Counter stained with hematoxylin.

Product Discontinued

Glutaminase Antibody (SN68-09) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human Glutaminase aa 50-100. (SwissProt: O94925 Human; SwissProt: D3Z7P3 Mouse; SwissProt: P13264 Rat)
• Localization: Cytoplasm, Mitochondrion.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GLS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Glutaminase Antibody (SN68-09)

• AAD20
• EC 3.5.1.2
• GLS
• GLS1DKFZp686O15119
• glutaminase C
• glutaminase kidney isoform, mitochondrial
• Glutaminase
• glutaminase, phosphate-activated
• K-Glutaminase
• KIAA0838FLJ10358
• L-Glutamine Amidohydrolase

Background

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLS