Glut1 Antibody


Western Blot: Glut1 Antibody [NB300-666] - Western blot analysis of 786-mock cells stably expressing shRNA constructs. For HIF2A (NB100-480) and HIF1B (NB100-124) analysis, nuclear extracts were generated and analyzed. more
Western Blot: Glut1 Antibody [NB300-666] - WB analysis of purified Glucose Transporter (GLUT1) with GLUT1 antibody at a dilution of 1:2000.
Western Blot: Glut1 Antibody [NB300-666] - Upregulation of Glut1 (NB300-666) and Glut3 (NBP2-66872) expression by Aqp9 gene deletion and ONC. Western blots for Glut1 and Glut3 protein expression levels in WT and Aqp9 KO more
Western Blot: Glut1 Antibody [NB300-666] - Rat brain cortical extracts (10 ug total protein per lane) on 10% SDS-PAGE gel. Antibody diluted 1:2000.
CNS vascular defects in gpr124 mutants. (G) Vasculature of wild-type and gpr124 mutant adults. Single plane confocal image of the vascular network (upper panels: scale bar, 100 um) and immunostaining for Slc2a1 and Pgp more

Product Details

Reactivity Hu, Mu, Rt, ZeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP

Order Details

Glut1 Antibody Summary

This Glut1 antibody is made against a synthetic peptide conjugated to KLH, corresponding to amino acids 478-492 of Human Glucose Transporter GLUT1. Peptide was covalently modified. Sequence: PEELFHPLGADSQV
Integral membrane protein. Localizes primarily at the cell surface (by similarity).
Plasma Membrane Marker
Glucose Transporter GLUT1
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA 1:12000
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Immunoprecipitation 1:200
  • Western Blot 1:5000
Application Notes
Immunoprecipitation: 2 uL will immunoprecipitate 80-85% Glut1 from rat hippocampal membranes. By Western Blot, a 50 kDa band is seen (predicted MW is 54.1 kDa). Optimal dilutions should be determined by the end user.
Theoretical MW
54.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 3.5
NB300-666 in the following applications:

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NB300-666 in the following applications:

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 26657775).

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Tris/Glycine buffer (pH 7.4 - 7.8), HEPES, 0.5% BSA, 30% glycerol
0.02% Sodium Azide
Immunogen affinity purified

Alternate Names for Glut1 Antibody

  • Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis)
  • CSE
  • DYT17
  • DYT18
  • DYT9
  • EIG12
  • Glucose transporter type 1, erythrocyte/brain
  • Glut1
  • GLUT-1
  • HepG2 glucose transporter
  • Human T-Cell Leukemia Virus (I and II) Receptor
  • MGC141895
  • MGC141896
  • PED
  • SLC2A1
  • solute carrier family 2 (facilitated glucose transporter), member 1
  • Solute Carrier Family 2 Member 1
  • solute carrier family 2, facilitated glucose transporter member 1


Glucose transporter 1 (GLUT1) or solute carrier family 2 (SLC2A1) is a member of the GLUT family of monosaccharides and polyols transporters. GLUT proteins transport glucose across cellular membranes through facilitative mechanisms and play a key role in glucose homeostasis (1). Fourteen GLUT proteins have been identified in the human, which are encoded by SLC2A genes 1-14 and are broadly expressed in many cell types and tissues. GLUT family members differ in sequence homology, substrate specificity and expression patterns. Based on sequence homology, GLUT family members are classified into Class I (GLUT1, 2, 3, 4, and GLUT14), Class II (GLUT5, 7, 9, and 11), and Class III (GLUT6, 8, 10, 12 and 13) (1). Structurally, GLUT transporters are integral membrane glycoproteins consisting of 12 membrane spanning helical domains, a single N-linked glycosylation site, and having cytoplasmic facing carboxy and amino terminal domains (2).

GLUT1 (Human glycosylated form theoretical molecular weight 55kDa) functions primarily as a glucose transporter but can transport other substrates including mannose, galactose and glucosamine across the membrane (3). Like other GLUT family members, GLUT1 is broadly expressed, nevertheless it is the predominant glucose transporter expressed in red blood cells and brain endothelial cells (1). SLC2A1 mutations underscore the autosomal dominant disorder GLUT1 deficiency syndrome (GLUTI-DS) which is characterized by low glucose levels in the brain or hypoglycorrhachia due to insufficient glucose transport across the blood brain barrier (2, 4, 5). Phenotypically, GLUT1-DS is characterized by early onset seizures, neurologic developmental delay, microcephaly, and ataxia (4). GLUT1 is highly expressed in the endothelium of cutaneous vascular lesions and serves as a marker for the diagnosis of juvenile or infantile hemangiomas (6).


1. Augustin, R. (2010). The protein family of glucose transport facilitators: It's not only about glucose after all. IUBMB Life.

2. Mueckler, M., & Thorens, B. (2013). The SLC2 (GLUT) family of membrane transporters. Molecular Aspects of Medicine.

3. Stein, W. D., & Litman, T. (2015). Carrier-Mediated Transport. In Channels, Carriers, and Pumps.

4. Pearson, T. S., Akman, C., Hinton, V. J., Engelstad, K., & De Vivo, D. C. (2013). Phenotypic spectrum of glucose transporter type 1 deficiency syndrome (Glut1 DS). Current Neurology and Neuroscience Reports.

5. Messana, T., Russo, A., Vergaro, R., Boni, A., Santucci, M., & Pini, A. (2018). Glucose transporter type 1 deficiency syndrome: Developmental delay and early-onset ataxia in a novel mutation of the SLC2A1 gene. Journal of Pediatric Neurosciences.

6. van Vugt, L. J., van der Vleuten, C. J. M., Flucke, U., & Blokx, W. A. M. (2017). The utility of GLUT1 as a diagnostic marker in cutaneous vascular anomalies: A review of literature and recommendations for daily practice. Pathology Research and Practice.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Pm
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Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC
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Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
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Applications: ICC/IF, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu, Mu, Rt
Applications: DB, ELISA, ICC/IF, IHC-P, IP, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IP, WB
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Publications for Glut1 Antibody (NB300-666)(29)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Fish, Zebrafish.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB300-666 Applications Species
Finicle BT Targeting endolysosomal trafficking with synthetic sphingolipid analogs to improve the delivery of oligonucleotide therapeutics Thesis 2023-01-01 (ICC/IF) ICC/IF
Parab S, Card OA, Chen Q et al. Local angiogenic interplay of Vegfc/d and Vegfa controls brain region-specific emergence of fenestrated capillaries eLife 2023-05-16 [PMID: 37191285] (IHC-P, Fish)

1:200 IHC-P dilution
IHC-P Fish
Fetsko AR, Sebo DJ, Taylor MR Brain endothelial cells acquire blood-brain barrier properties in the absence of Vegf-dependent CNS angiogenesis Developmental biology 2022-12-09 [PMID: 36502932] (IHC, Zebrafish) IHC Zebrafish
Langbein LE, El Hajjar R, He S et al. BAP1 maintains HIF-dependent interferon beta induction to suppress tumor growth in clear cell renal cell carcinoma Cancer letters 2022-08-20 [PMID: 35995140] (WB, Human)

Dilutions: 1:2000
WB Human
Harder I, MUnchhalfen M, Andrieux G et al. Dysregulated PI3K Signaling in B Cells of CVID Patients Cells 2022-01-28 [PMID: 35159274] (Human) Human
Kanan Y, Hackett SF, Taneja K et al. Oxidative stress-induced alterations in retinal glucose metabolism in Retinitis Pigmentosa Free radical biology & medicine 2022-03-01 [PMID: 35134532] (WB, Mouse) WB Mouse
Laitakari A, Huttunen R, Kuvaja P et al. Systemic long-term inactivation of hypoxia-inducible factor prolyl 4-hydroxylase 2 ameliorates aging-induced changes in mice without affecting their life span FASEB J. 2020-02-25 [PMID: 32100354] (WB, Mouse) WB Mouse
Chowdhury A, Aich A, Jain G et al. Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1a Expression in Hypoxia. Cell Rep. 2018-10-16 [PMID: 30332638] (WB, Mouse) WB Mouse
Miyazawa H, Yamamoto M, Yamaguchi Y et al. Mammalian embryos show metabolic plasticity toward the surrounding environment during neural tube closure Genes Cells 2018-08-08 [PMID: 30088697] (Rat) Rat
Finicle BT, Ramirez MU, Liu G et al. Sphingolipids inhibit endosomal recycling of nutrient transporters by inactivating ARF6. J Cell Sci 2018-06-25 [PMID: 29848659]
Show All 29 Publications.

Reviews for Glut1 Antibody (NB300-666) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB300-666:
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Images Ratings Applications Species Date Details
Western Blot Glut1 NB300-666
reviewed by:
Verified Customer
WB Human 10/04/2015


ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
reviewed by:
Verified Customer
WB Human 12/23/2014


ApplicationWestern Blot
Sample TestedSee PMID 24260413

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Blogs on Glut1.

Deficiency of GluT1 leads to neurological problems while excess is involved in cancers
By Jamshed Arslan, Pharm. D., PhD. What Are GluTs?Mammalian cell metabolism is incomplete without glucose   . Glucose is a monosaccharide that is transported to the cells through facilitative diffusion, a ...  Read full blog post.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Forecasting and Targeting a Rare Cancer with Hypoxia-Inducible Factor
By Jamshed Arslan Pharm.D. Cancers of nerve, adipose, and other soft tissues are called soft tissue sarcomas (STS). Malignant peripheral nerve sheath tumor (MPNST) is an example of a rare and hard-to-treat STS; eve...  Read full blog post.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

Glucose Transporter 1 (GLUT1): a Key Metabolic Neuronal Player
Glucose is the principal fuel source for the brain and GLUT1 is the only vehicle by which glucose enters the brain. In case of GLUT1 deficiency, the risk of clinical manifestations is increased in infancy and childhood, when the brain glucose demand i...  Read full blog post.

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Recent Reviews


Verified Customer
Application: WB
Species: Human

Verified Customer
Application: WB
Species: Human


Gene Symbol SLC2A1