Glut1 Antibody

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Western Blot: Glut1 Antibody [NB300-666] - Western blot analysis of 786-mock cells stably expressing shRNA constructs. For HIF2A (NB100-480) and HIF1B (NB100-124) analysis, nuclear extracts were generated and analyzed. ...read more
Western Blot: Glut1 Antibody [NB300-666] - WB analysis of purified Glucose Transporter (GLUT1) with GLUT1 antibody at a dilution of 1:2000.
Western Blot: Glut1 Antibody [NB300-666] - Rat brain cortical extracts (10 ug total protein per lane) on 10% SDS-PAGE gel. Antibody diluted 1:2000.

Product Details

Summary
Reactivity Hu, Mu, Rt, ZeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Glut1 Antibody Summary

Immunogen
This Glut1 antibody is made against a synthetic peptide conjugated to KLH, corresponding to amino acids 478-492 of Human Glucose Transporter GLUT1. Peptide was covalently modified. Sequence: PEELFHPLGADSQV
Localization
Integral membrane protein. Localizes primarily at the cell surface (by similarity).
Marker
Plasma Membrane Marker
Specificity
Glucose Transporter GLUT1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC2A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • ELISA 1:12000
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Immunoprecipitation 1:200
Application Notes
Immunoprecipitation: 2 uL will immunoprecipitate 80-85% Glut1 from rat hippocampal membranes. By Western Blot, a 50 kDa band is seen (predicted MW is 54.1 kDa). Optimal dilutions should be determined by the end user.
Theoretical MW
54.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Glut1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 2 Reviews rated 3.5
using
NB300-666 in the following applications:

Publications
Read Publications using
NB300-666 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    8 publications

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 26657775).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris/Glycine buffer (pH 7.4 - 7.8), HEPES, 0.5% BSA, 30% glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Glut1 Antibody

  • Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis)
  • CSE
  • DYT17
  • DYT18
  • DYT9
  • EIG12
  • Glucose transporter type 1, erythrocyte/brain
  • Glut1
  • GLUT-1
  • GLUT1DS
  • HepG2 glucose transporter
  • HTLVR
  • Human T-Cell Leukemia Virus (I and II) Receptor
  • MGC141895
  • MGC141896
  • PED
  • SLC2A1
  • solute carrier family 2 (facilitated glucose transporter), member 1
  • Solute Carrier Family 2 Member 1
  • solute carrier family 2, facilitated glucose transporter member 1

Background

Glucose transporter 1 (GLUT1) or solute carrier family 2 (SLC2A1) is a member of the GLUT family of monosaccharides and polyols transporters. GLUT proteins transport glucose across cellular membranes through facilitative mechanisms and play a key role in glucose homeostasis (1). Fourteen GLUT proteins have been identified in the human, which are encoded by SLC2A genes 1-14 and are broadly expressed in many cell types and tissues. GLUT family members differ in sequence homology, substrate specificity and expression patterns. Based on sequence homology, GLUT family members are classified into Class I (GLUT1, 2, 3, 4, and GLUT14), Class II (GLUT5, 7, 9, and 11), and Class III (GLUT6, 8, 10, 12 and 13) (1). Structurally, GLUT transporters are integral membrane glycoproteins consisting of 12 membrane spanning helical domains, a single N-linked glycosylation site, and having cytoplasmic facing carboxy and amino terminal domains (2).

GLUT1 (Human glycosylated form theoretical molecular weight 55kDa) functions primarily as a glucose transporter but can transport other substrates including mannose, galactose and glucosamine across the membrane (3). Like other GLUT family members, GLUT1 is broadly expressed, nevertheless it is the predominant glucose transporter expressed in red blood cells and brain endothelial cells (1). SLC2A1 mutations underscore the autosomal dominant disorder GLUT1 deficiency syndrome (GLUTI-DS) which is characterized by low glucose levels in the brain or hypoglycorrhachia due to insufficient glucose transport across the blood brain barrier (2, 4, 5). Phenotypically, GLUT1-DS is characterized by early onset seizures, neurologic developmental delay, microcephaly, and ataxia (4). GLUT1 is highly expressed in the endothelium of cutaneous vascular lesions and serves as a marker for the diagnosis of juvenile or infantile hemangiomas (6).

References

1. Augustin, R. (2010). The protein family of glucose transport facilitators: It's not only about glucose after all. IUBMB Life. https://doi.org/10.1002/iub.315

2. Mueckler, M., & Thorens, B. (2013). The SLC2 (GLUT) family of membrane transporters. Molecular Aspects of Medicine. https://doi.org/10.1016/j.mam.2012.07.001

3. Stein, W. D., & Litman, T. (2015). Carrier-Mediated Transport. In Channels, Carriers, and Pumps. https://doi.org/10.1016/b978-0-12-416579-3.00004-6

4. Pearson, T. S., Akman, C., Hinton, V. J., Engelstad, K., & De Vivo, D. C. (2013). Phenotypic spectrum of glucose transporter type 1 deficiency syndrome (Glut1 DS). Current Neurology and Neuroscience Reports. https://doi.org/10.1007/s11910-013-0342-7

5. Messana, T., Russo, A., Vergaro, R., Boni, A., Santucci, M., & Pini, A. (2018). Glucose transporter type 1 deficiency syndrome: Developmental delay and early-onset ataxia in a novel mutation of the SLC2A1 gene. Journal of Pediatric Neurosciences. https://doi.org/10.4103/JPN.JPN_169_17

6. van Vugt, L. J., van der Vleuten, C. J. M., Flucke, U., & Blokx, W. A. M. (2017). The utility of GLUT1 as a diagnostic marker in cutaneous vascular anomalies: A review of literature and recommendations for daily practice. Pathology Research and Practice. https://doi.org/10.1016/j.prp.2017.04.023

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Ze
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP

Publications for Glut1 Antibody (NB300-666)(23)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Zebrafish.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
WB
(8)
All Applications
Filter By Species
Human
(4)
Mouse
(5)
Rat
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NB300-666 Applications Species
Laitakari A, Huttunen R, Kuvaja P et al. Systemic long-term inactivation of hypoxia-inducible factor prolyl 4-hydroxylase 2 ameliorates aging-induced changes in mice without affecting their life span FASEB J. Feb 25 2020 [PMID: 32100354] (WB, Mouse) WB Mouse
Chowdhury A, Aich A, Jain G et al. Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1a Expression in Hypoxia. Cell Rep. Oct 16 2018 [PMID: 30332638] (WB, Mouse) WB Mouse
Miyazawa H, Yamamoto M, Yamaguchi Y et al. Mammalian embryos show metabolic plasticity toward the surrounding environment during neural tube closure Genes Cells Aug 8 2018 [PMID: 30088697] (Rat) Rat
Finicle BT, Ramirez MU, Liu G et al. Sphingolipids inhibit endosomal recycling of nutrient transporters by inactivating ARF6. J Cell Sci 2018 Jun 25 [PMID: 29848659]
Kim SM, Roy SG, Chen B et al. Targeting cancer metabolism by simultaneously disrupting parallel nutrient access pathways J. Clin. Invest. Sep 26 2016 [PMID: 27669461] (ICC/IF, Mouse) ICC/IF Mouse
Joyal JS, Sun Y, Gantner ML et al. Retinal lipid and glucose metabolism dictates angiogenesis through the lipid sensor Ffar1. Nat Med 2016 Apr [PMID: 26974308]
Ulrich F, Carretero-Ortega J, MenEndez J et al. Reck enables cerebrovascular development by promoting canonical Wnt signaling Development. 2015 Dec 31 [PMID: 26657775] (IHC, Zebrafish) IHC Zebrafish
Lin AP, Abbas S, Kim SW et al. D2HGDH regulates alpha-ketoglutarate levels and dioxygenase function by modulating IDH2 Nat Commun. 2015 Jul 16 [PMID: 26178471] (WB, Human) WB Human
Vanhollebeke B, Stone OA, Bostaille N et al. Tip cell-specific requirement for an atypical Gpr124- and Reck-dependent Wnt/beta-catenin pathway during brain angiogenesis. Elife 2015 [PMID: 26051822]
Zhang T, Niu X, Liao L et al. The Contributions of HIF-Target Genes to Tumor Growth in RCC. PLoS One. 2013 Nov 18 [PMID: 24260413] (WB, Human) WB Human
Show All 23 Publications.

Reviews for Glut1 Antibody (NB300-666) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB300-666:
Filter by Applications
WB
(2)
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Human
(2)
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Images Ratings Applications Species Date Details
Western Blot Glut1 NB300-666
Enlarge
3
reviewed by:
Anonymous
WB Human 10/04/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA934V
Secondary Concentration1:2000

Details

Detection NotesRegular Western blot Protocol
  4
reviewed by:
Anonymous
WB Human 12/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24260413
SpeciesHuman

Blocking

Blocking DetailsSee PMID 24260413

Details

Detection NotesSee PMID 24260413

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 24260413.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Glut1 Antibody (NB300-666) (0)

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Control Lysate(s)

Secondary Antibodies

 

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Blogs on Glut1.

Deficiency of GluT1 leads to neurological problems while excess is involved in cancers
By Jamshed Arslan, Pharm. D., PhD. What Are GluTs?Mammalian cell metabolism is incomplete without glucose   . Glucose is a monosaccharide that is transported to the cells through facilitative diffusion, a...  Read full blog post.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Forecasting and Targeting a Rare Cancer with Hypoxia-Inducible Factor
By Jamshed Arslan Pharm.D. Cancers of nerve, adipose, and other soft tissues are called soft tissue sarcomas (STS). Malignant peripheral nerve sheath tumor (MPNST) is an example of a rare and hard-to-treat STS; eve...  Read full blog post.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

Glucose Transporter 1 (GLUT1): a Key Metabolic Neuronal Player
Glucose is the principal fuel source for the brain and GLUT1 is the only vehicle by which glucose enters the brain. In case of GLUT1 deficiency, the risk of clinical manifestations is increased in infancy and childhood, when the brain glucose demand i...  Read full blog post.

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Recent Reviews

3.5
5
0
4
1
3
1
2
0
1
0

Anonymous
10/04/2015
Application: WB
Species: Human

 
Anonymous
12/23/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol SLC2A1
Entrez
Uniprot