GLUD1/GLUD2 Antibody (848209) [Unconjugated]

Images

 
Western blot shows lysates of human brain (cortex) tissue, human testis tissue, mouse brain (cortex) tissue, and rat brain (cortex) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat ...read more
GLUD1/GLUD2 was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Human/Mouse/Rat GLUD1/GLUD2 Monoclonal Antibody (Catalog # MAB8027) at 0.2 µg/mL for 1 hour at room temperature ...read more
Simple Western lane view shows lysates of human brain (motor cortex) tissue, loaded at 0.5 mg/mL. A specific band was detected for GLUD2 at approximately 58 kDa (as indicated) using 2 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clone
848209
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

GLUD1/GLUD2 Antibody (848209) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GLUD1/GLUD2
Ser54-Thr558
Accession # P49448
Specificity
Detects human, mouse and rat GLUD1/GLUD2 in ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GLUD2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Simple Western 2 ug/mL
  • Immunohistochemistry 0.2-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLUD1/GLUD2 Antibody (848209) [Unconjugated]

  • GLUD1/GLUD2

Background

Glutamate dehydrogenase 2 (GLUD2 or GDH2) is an approximately 58 kDa mitochondrial enzyme that catalyzes the reversible interconversion of glutamate to alpha‑ketoglutarate and ammonia. This activity is important in glutamine and ammonia metabolism as well as glutamatergic signal transduction. In contrast to the widely expressed GLUD1, GLUD2 expression is restricted to testicular Sertoli and Leydig cells, brain astrocytes, and renal tubule epithelial cells. GLUD2 and GLUD1 are synthesized with N-terminal mitochondrial transit peptides. Within amino acids 58-558, human GLUD2 and GLUD1 share 97% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional GLUD1/GLUD2 Products

Array MAB8027

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Bioinformatics

Gene Symbol GLUD2
Uniprot