GLUD1/GLUD2 Antibody (848209) [Unconjugated]

Western blot shows lysates of human brain (cortex) tissue, human testis tissue, mouse brain (cortex) tissue, and rat brain (cortex) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat GLUD1/GLUD2 Monoclonal Antibody (Catalog # MAB8027) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GLUD2 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GLUD1/GLUD2 was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Human/Mouse/Rat GLUD1/GLUD2 Monoclonal Antibody (Catalog # MAB8027) at 0.2 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to astrocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of human brain (motor cortex) tissue, loaded at 0.5 mg/mL. A specific band was detected for GLUD2 at approximately 58 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Mouse/Rat GLUD1/GLUD2 Monoclonal Antibody (Catalog # MAB8027). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clone: 848209
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GLUD1/GLUD2 Antibody (848209) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human GLUD1/GLUD2
  Ser54-Thr558
  Accession # P49448
• Specificity: Detects human, mouse and rat GLUD1/GLUD2 in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GLUD2

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL
  • Simple Western 2 ug/mL
  • Immunohistochemistry 0.2-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLUD1/GLUD2 Antibody (848209) [Unconjugated]

• GLUD1/GLUD2

Background

Glutamate dehydrogenase 2 (GLUD2 or GDH2) is an approximately 58 kDa mitochondrial enzyme that catalyzes the reversible interconversion of glutamate to alpha‑ketoglutarate and ammonia. This activity is important in glutamine and ammonia metabolism as well as glutamatergic signal transduction. In contrast to the widely expressed GLUD1, GLUD2 expression is restricted to testicular Sertoli and Leydig cells, brain astrocytes, and renal tubule epithelial cells. GLUD2 and GLUD1 are synthesized with N-terminal mitochondrial transit peptides. Within amino acids 58-558, human GLUD2 and GLUD1 share 97% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLUD2
• Uniprot:
  • P49448()