Glutamate Dehydrogenase Antibody (848209) [Alexa Fluor® 350]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 848209
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

Glutamate Dehydrogenase Antibody (848209) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human GLUD1/GLUD2
  Ser54-Thr558
  Accession # P49448
• Specificity: Detects human, mouse and rat GLUD1/GLUD2 in ELISAs and Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glutamate Dehydrogenase Antibody (848209) [Alexa Fluor® 350]

• EC 1.4.1
• EC 1.4.1.3
• GDH 1
• GDH
• GDH1
• GLUD
• glutamate dehydrogenase (NAD(P)+)
• Glutamate Dehydrogenase 1
• glutamate dehydrogenase 1, mitochondrial
• MGC132003

Background

Glutamate dehydrogenase 2 (GLUD2 or GDH2) is an approximately 58 kDa mitochondrial enzyme that catalyzes the reversible interconversion of glutamate to alpha‑ketoglutarate and ammonia. This activity is important in glutamine and ammonia metabolism as well as glutamatergic signal transduction. In contrast to the widely expressed GLUD1, GLUD2 expression is restricted to testicular Sertoli and Leydig cells, brain astrocytes, and renal tubule epithelial cells. GLUD2 and GLUD1 are synthesized with N-terminal mitochondrial transit peptides. Within amino acids 58-558, human GLUD2 and GLUD1 share 97% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.